Interaction of myosin subfragment 1 with Cibacron Blue F3GA.
Interaction of myosin subfragment 1 with Cibacron Blue F3GA.
复制标题
肌球蛋白亚片段 1 与 Cibacron Blue F3GA 的相互作用。
作者：
Reisler,E;Liu,J
Materials and Methods-Chymotrypsin, ADP, and ATP were obtained from Sigma Chemical Co.(St. Louis, MO).[3H] ADP was purchased from New England Nuclear. Cibacron Blue F3GA and Procion Blue MX-R (dichlorotriazine dye) were obtained from Polysciences (Warrington, PA). 2-Anthraquinonesulfonic acid was the product of Eastman Kodak Co.(Rochester, NY). Cibacron Blue F3GA covalently attached to cross-linked agarose was purchased from Bio-Rad Laboratories. Ultrapure guanidine hydrochloride was obtained fromSchwarz/Mann Co.(Orangeburg, NY), and p-phenylenedimaleimide was the
