ONE OF 3 NUCLEAR-LOCALIZATION SIGNALS OF MAIZE ACTIVATOR (AC) TRANSPOSASE OVERLAPS THE DNA-BINDING DOMAIN
ONE OF 3 NUCLEAR-LOCALIZATION SIGNALS OF MAIZE ACTIVATOR (AC) TRANSPOSASE OVERLAPS THE DNA-BINDING DOMAIN
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DOI：
10.1046/j.1365-313x.1995.7030441.x
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发表时间：
1995-03-01
期刊：
影响因子：
7.2
通讯作者：
KUNZE, R
中科院分区：
文献类型：
--
作者：
BOEHM, U;HEINLEIN, M;KUNZE, R
The nuclear localization sequences (NLSs) of the Ac transposase (TPase) protein have been characterized by indirect immunofluorescence detection of TPase deletion derivatives and TPase/beta-glucuronidase (GUS) fusion proteins in transiently transfected Petunia cells. The TPase contains three NLSs near its amino-terminal end, NLS(44-62), NLS(159-178) and NLS(174-206), each of which is sufficient to redirect GUS to the nucleus. Deletion of the N-terminal 102 TPase residues including NLS(44-62) results in strongly reduced nuclear import of the truncated TPase. NLS(44-62) and NLS(159-178) are bipartite NLSs, whereas the structure of NLS(174-206) does not allow a classification into one of the three major NLS categories. NLS(174-206) overlaps with the basic DNA-binding domain of TPase. A substitution of two amino acids in this segment (His(191)-->Arg and Arg(193)-->His) results in a total loss of DNA-binding activity, but retains reduced NLS activity. Accordingly, the two functions can be separated. In addition, we show that a NLS-deficient 71 kDa TPase derivative is co-imported into the nucleus in the presence of wild-type TPase.
