High prevalence of VIM-4 and NDM-1 metallo-β-lactamase among carbapenem-resistant Enterobacteriaceae
High prevalence of VIM-4 and NDM-1 metallo-β-lactamase among carbapenem-resistant Enterobacteriaceae
复制标题
DOI：
10.1099/jmm.0.059915-0
复制
发表时间：
2013-08-01
影响因子：
3
通讯作者：
Poirel, Laurent
中科院分区：
文献类型：
--
作者：
Jamal, Wafaa;Rotimi, Vincent O.;Poirel, Laurent
本研究的目的是确定科威特Mubarak Al Kabeer医院医院感染住院患者中多药耐药肠杆菌科分离株对碳青霉烯类耐药的机制。2009年4月至2011年2月，从不同病房和重症监护病房的住院患者中分离出14株耐碳青霉烯类肠杆菌。采用E-test法测定抗生素敏感性。编码β -内酰胺酶的基因通过特异性PCR扩增、测序和偶联实验进行了表征。利用表型和分子方法鉴定所有分离株为金属β -内酰胺酶（MBL）产生菌。14株分离菌中有11株产生VIM-4（6株肺炎克雷伯菌、3株大肠埃希菌、1株阴沟肠杆菌和1株氧化克雷伯菌）。三个肺炎克雷伯菌分离株产生MBL NDM-1，并共同产生编码AmpC CMY-4的质粒。产生vim -4的分离株共同产生广谱β -内酰胺酶，包括CTX-M-15和一些SHV衍生物。通过对6株选定菌株的偶联研究，VIM-4基因不具有可转移性。我们在这里证明了在科威特最大的教学医院中出现了产生VIM-4和ndm -1的分离株。
The purpose of this study was to identify the mechanisms leading to carbapenem resistance among multidrug-resistant Enterobacteriaceae isolates recovered from hospitalized patients with nosocomial infections in Mubarak Al Kabeer Hospital, Kuwait. Fourteen carbapenem-resistant Enterobacteriaceae isolates were obtained from inpatients in different wards and intensive care units between April 2009 and February 2011. Antibiotic susceptibilities were determined using the E-test method. Genes encoding beta-lactamases were characterized by specific PCR amplification, sequencing and conjugation assays. All isolates were identified as metallo-beta-lactamase (MBL) producers using phenotypic and molecular methods. Eleven of the 14 isolates produced VIM-4 (six Klebsiella pneumoniae, three Escherichia coli, one Enterobacter cloacae and one Klebsiella oxytoca). Three K. pneumoniae isolates produced the MBL NDM-1 and co-produced the plasmid-encoded AmpC CMY-4. The VIM-4-producing isolates co-produced extended-spectrum beta-lactamases including CTX-M-15 and some SHV derivatives. The VIM-4 gene was not transferable by conjugation studies of six selected strains. We demonstrated here the emergence of VIM-4- and NDM-1-producing isolates in the largest teaching hospital in Kuwait.