Identification of QTLs controlling low-temperature germination of the East European rice (Oryza sativa L.) variety Maratteli
Identification of QTLs controlling low-temperature germination of the East European rice (Oryza sativa L.) variety Maratteli
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DOI：
10.1007/s10681-015-1531-z
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发表时间：
2016-01-01
期刊：
影响因子：
1.9
通讯作者：
Akagi, Hiromori
中科院分区：
文献类型：
--
作者：
Satoh, Tomohiro;Tezuka, Kouichi;Akagi, Hiromori
低温发芽力是水稻直播成苗的重要性状之一。本研究的目的是鉴定在低温下发芽高和快的基因，目的是培育新的水稻品种直播栽培。我们确定了4个数量性状位点（QTL）负责发芽性能在低温下在东欧水稻品种，马拉泰利。3号染色体上的qLTG 3 -1、qLTG 3 -2和11号染色体上的qLTG 11 -1等位基因使种子在低温下发芽率增加。相反，1号染色体上qLTG 1 -1的Maratteli等位基因降低了该速率。近等基因系（NILs）与秋田小町遗传背景窝藏qLTG 3 -1和qLTG 1 -1 -1等位基因从马拉泰利加速发芽和近等基因系与qLTG 1 -1延迟发芽在低温下，分别导致增强或抑制发芽率。这些QTL改变了秋田小町的发芽特性。因此，Maratteli是一个更好的基因来源，为改善发芽在低温下。我们的结论是，低温发芽力的各种日本品种在这一字符的劣势，可以通过分子标记辅助选择，而不修改其他上级性状。这些近等基因也可能有助于揭示种子萌发阶段耐冷性的分子机制。
Germinability under low temperature is one of the most important traits in seedling establishment in direct-sowing culture of rice. The objective of this study was the identification of genes responsible for higher and faster germination under low temperature, with the aim of breeding new rice varieties for direct-sowing culture. We identified four quantitative trait loci (QTLs) responsible for germination properties under low temperature in an East European rice variety, Maratteli. Maratteli-derived alleles at qLTG3-1 and qLTG3-2 on chromosome 3 and qLTG11-1 on chromosome 11 increased germination rate under low temperature. In contrast, the Maratteli allele at qLTG1-1 on chromosome 1 reduced the rate. Near-isogenic lines (NILs) with the Akitakomachi genetic background harboring qLTG3-1 and qLTG11-1 alleles from Maratteli accelerated germination and a NIL with qLTG1-1 delayed germination under low temperature, resulting in enhanced or suppressed germination rates, respectively. These QTLs altered the germination profile of Akitakomachi. Hence, Maratteli is a better source of genes for the improvement of germination under low temperature. We conclude that the low temperature germinability of various Japanese varieties inferior in this character can be improved by marker-assisted selection without modification of other superior traits. These NILs may also contribute to uncovering the molecular mechanisms of cold tolerance at the germination stage.