Characterization of acrylic polyamide plastic embolization particles in vitro and in human tissue sections by light microscopy, infrared microspectroscopy and scanning electron microscopy with energy dispersive X-ray analysis
Characterization of acrylic polyamide plastic embolization particles in vitro and in human tissue sections by light microscopy, infrared microspectroscopy and scanning electron microscopy with energy dispersive X-ray analysis
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DOI：
10.1038/modpathol.3800595
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发表时间：
2006-07-01
期刊：
影响因子：
7.5
通讯作者：
Mullick, Florabel G.
中科院分区：
文献类型：
--
作者：
Murakata, Linda A.;Lewin-Smith, Michael R.;Mullick, Florabel G.
血管栓塞术是治疗肿瘤和血管病变的一种行之有效的做法。各种材料(胶原蛋白、葡聚糖和三丙烯基聚合物明胶)的圆形珠子(微球)被开发出来，以解决早期版本的栓塞物遇到的问题。我们对2例子宫和1例肝脏标本进行了组织化学、傅里叶变换红外光谱和扫描电子显微镜能量色散X射线分析，并检测了一种对照栓塞物。苏木精-伊红染色的组织切片显示多个病灶，血管内异物不明，管腔纤维性闭塞。只有一例有既往栓塞的临床病史，但没有具体说明使用的材料。提交了一例用于鉴定“寄生虫”的病例。天狼星红、粘液红染色阳性，Masson‘s三色染色、Movat五色染色不同，高碘酸席夫淀粉酶染色中央不阳性。对所有三个案例材料的红外光谱分析表明，这是丙烯酸聚酰胺塑料的光谱。EmboGold(TM)的对照样品显示了与三个组织样品类似的红外显微光谱。扫描电子显微镜和能量色散X射线分析表明，组织切片和所选参考材料的元素组成存在一些差异。据我们所知，这是首次在体外和人体组织切片中用扫描电子显微镜和能量色散X射线分析对丙烯酸聚酰胺塑料栓塞物进行红外光谱分析。在缺乏适当临床信息的情况下，通过这些方法和/或一组特殊染色进行鉴定可能会帮助不熟悉这种材料的光学显微镜外观的病理学家。
Vascular embolization is a well-established practice for the treatment of tumors and vascular lesions. Rounded beads (microspheres) of various materials (collagen, dextran and trisacryl-polymer-gelatin) were developed to solve problems encountered with earlier versions of embolic material. We performed histochemistry, Fourier transform infrared microspectroscopy and scanning electron microscopy with energy dispersive X-ray analysis on two uterine and one hepatic specimen with unidentified intravascular foreign material, and examined a reference embolization product for comparison. The hematoxylin and eosin stained tissue sections showed multiple foci with unidentified intravascular foreign material and fibrous obliteration of vessel lumens. Only one case had a clinical history of previous embolization but without specifying the material used. One case was submitted for identification of a 'parasite'. The material stained positively with Sirius red and mucicarmine, variably with Masson's trichrome stain and Movat pentachrome, and did not stain centrally with periodic acid Schiff with diastase. Infrared spectrophotometric analysis of the material from all three cases demonstrated the spectrum of acrylic polyamide plastic. A control sample of EmboGold (TM) exhibited infrared microspectroscopic spectra similar to the three tissue specimens. Analysis by scanning electron microscopy with energy dispersive X-ray analysis demonstrated some differences in elemental composition between the tissue sections and the selected reference material. To our knowledge, this is the first report of infrared spectrophotometric analysis with scanning electron microscopy with energy dispersive X-ray analysis of an acrylic polyamide plastic embolization product both in vitro and in human histologic tissue sections. In cases lacking appropriate clinical information, identification by these methods and/or a panel of special stains may assist pathologists unfamiliar with this material's light microscopic appearance.