Thermal resistance traits of the clonal silver crucian carp Carassius langsdorfii and evaluation of these traits using primary culture cells
Thermal resistance traits of the clonal silver crucian carp Carassius langsdorfii and evaluation of these traits using primary culture cells
复制标题
无性系银鲫耐热性状及原代培养细胞评价
DOI：
10.1046/j.1444-2906.2002.00528.x
复制
发表时间：
2002
影响因子：
1.9
通讯作者：
N. Taniguchi
中科院分区：
文献类型：
--
作者：
K. Sakamoto;Worawut Koedprang;M. Nakajima;N. Taniguchi
:
对银鲫3个无性系（SCC-1、SCC-2、SCC-3）的耐热性进行了研究。将在20°C下饲养的个体幼鱼暴露于36.5 ± 0.5°C的热应激，并测量其在此暴露期间的死亡时间。SCC-2和SCC-3鱼的死亡时间分别为66.6 ± 31.2和144.7 ± 49.8 min。相比之下，所有SCC-1鱼在这些条件下存活。此外，通过台盼蓝测定法在37、40或43°C下测定来自每个克隆的原代培养细胞的热敏感性。在所有处理条件下，SCC-1原代培养物的热敏感性均低于其他培养物。这些结果估计了体内和体外热阻之间的相关性。因此，利用原代培养细胞评价耐热性是一种有效的选择育种方法。
:
The thermal resistance traits of three clonal lines of the silver crucian carp Carassius langsdorfii (SCC-1, -2 and -3) were investigated. Individual juvenile fish reared at 20°C were exposed to thermal stress at 36.5 ± 0.5°C and their death times during this exposure were measured. The death times of SCC-2 and SCC-3 fish were 66.6 ± 31.2 and 144.7 ± 49.8 min, respectively. In contrast, all SCC-1 fish survived under these conditions. Furthermore, the thermal sensitivity of primary culture cells from each clone was determined at 37, 40 or 43°C by the Trypan blue assay. Under all treatment conditions, the thermal sensitivity of the SCC-1 primary cultures was lower than those of the others. These results estimated the correlation between the in vivo and in vitro thermal resistances. Therefore, the use of primary culture cells to evaluate thermal resistance could be a useful method of selection breeding.