Quality control of commercial Mediterranean oregano: Development of SCAR markers for the detection of the adulterants Cistus incanus L., Rubus caesius L. and Rhus coriaria L.
Quality control of commercial Mediterranean oregano: Development of SCAR markers for the detection of the adulterants Cistus incanus L., Rubus caesius L. and Rhus coriaria L.
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DOI：
10.1016/j.foodcont.2009.12.018
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发表时间：
2010-07-01
期刊：
影响因子：
6
通讯作者：
Bruni, Renato
中科院分区：
文献类型：
--
作者：
Marieschi, Matteo;Torelli, Anna;Bruni, Renato
最近的一项生药学调查显示，商业地中海牛至经常掺假与具有类似银灰色的干植物（Rims coriaria L ., Cistus spp.和Rubus spp.）。如果由熟练的掺假者进行，这种做法的检测几乎完全依赖于生药学分析，这是非常费力和耗时的，特别是当需要评估多批牛至时。序列特征扩增区标记（scar）是由3个RAPD标记发展而来的，这些RAPD标记是针对这些伪品的，目的是加快对Origanum批次的初步筛选，并允许对可疑样品进行先发制人的拒绝，从而缩小样品的数量，以便进行更仔细的生药学分析。SCAR引物扩增出预期大小的特定条带，允许检测到掺假植物的1%。这些扩增子相对较短的尺寸适合于分析从干燥和储存的商业材料中获得的潜在降解DNA。(C) 2010 Elsevier Ltd.版权所有。
A recent pharmacognostic survey showed a frequent adulteration of commercial Mediterranean oregano with dried plants having a similar silvery gray color (Rims coriaria L, Cistus spp. and Rubus spp.). If performed by skilled adulterators, the detection of this practice relies almost completely on pharmacognostic assays which are extremely laborious and time consuming, especially when multiple batches of oregano have to be evaluated. Sequence-characterized amplified regions markers (SCARs) were developed from three RAPD markers specific for these adulterants, in order to speed up the primary screening of Origanum batches and allow the pre-emptive rejection of suspect samples, thus narrowing the number of samples to be subjected to more careful pharmacognostic analyses. The SCAR primers gave rise to the amplification of specific bands of expected sizes which allowed the detection up to 1% of adulterating plants. The relatively short dimension of these amplicons is suitable for the analysis of potentially degraded DNA obtained from dried and stored commercial material. (C) 2010 Elsevier Ltd. All rights reserved.