CHANGES IN CARBOHYDRATE METABOLISM OF MITOGENICALLY STIMULATED HUMAN PERIPHERAL LYMPHOCYTES .3. STIMULATION BY TUBERCULIN AND ALLOGENEIC CELLS
CHANGES IN CARBOHYDRATE METABOLISM OF MITOGENICALLY STIMULATED HUMAN PERIPHERAL LYMPHOCYTES .3. STIMULATION BY TUBERCULIN AND ALLOGENEIC CELLS
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DOI：
10.1016/0014-4827(73)90498-9
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发表时间：
1973-01-01
影响因子：
3.7
通讯作者：
ROOS, D
中科院分区：
文献类型：
--
作者：
LOOS, JA;ROOS, D
已经开发了一种培养方法，其中培养基被连续透析。该方法允许以20· 106/ml的浓度培养人血淋巴细胞。6天后细胞回收率为60%，通过台盼蓝拒染法判断存活率为80%。在PPD或同种异体细胞存在下以这种方式培养的淋巴细胞显示出乳酸产生的刺激和无机磷酸盐掺入酸溶性核苷酸中。这些变化在3-4天后明显可检测到，并且在6天后甚至更大。有时糖酵解中间体的水平也会增加，但这些影响在6天后减弱。ATP + ADP水平和耗氧率保持不变。观察到的效应似乎是完全特异性的：当Mantoux阴性供体的淋巴细胞与PPD一起培养时，或者当来自HL-A相同兄弟姐妹的细胞在MLC中培养时，没有发现刺激。
A culture method has been developed in which the medium is continuously dialysed. This method permitted the culture of human blood lymphocytes at a concentration of 20· 106/ml. Cell recovery was 60% after 6 days with a viability of 80% as judged by trypan blue exclusion. Lymphocytes cultured in this way in the presence of PPD or allogeneic cells showed a stimulation of the lactate production and the incorporation of inorganic phosphate into acid-soluble nucleotides. These changes were clearly detectable after 3–4 days and were even greater after 6 days. Sometimes the levels of glycolytic intermediates increased as well, but these effects were diminished after 6 days. The levels of ATP + ADP and the rate of oxygen consumption remained unchanged. The observed effects appeared to be completely specific: no stimulation was found when lymphocytes of Mantoux negative donors were cultured with PPD or when cells from HL-A identical siblings were cultured in a MLC.