Endostyle Cell Recruitment as a Frame of Reference for Development and Growth in the Urochordate Oikopleura dioica
Endostyle Cell Recruitment as a Frame of Reference for Development and Growth in the Urochordate Oikopleura dioica
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Endostyle 细胞募集作为尾索动物 Oikopleura dioica 发育和生长的参考框架
DOI：
10.2307/25066650
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
E. Thompson
中科院分区：
文献类型：
--
作者：
C. Troedsson;Philippe Ganot;J. Bouquet;D. L. Aksnes;E. Thompson
在生长和生活史模型中，以及在分子和细胞生物学中，需要更准确的参照系来表征发育进程。在秀丽隐杆线虫中，细胞谱系的完整命运图谱提供了这样的参考标准。为了得到更广泛的应用，参照基准应更易于衡量，同时仍能提供强大的预测能力。为了实现这一目标，我们在细胞水平上分析了内柱的生长，并测量了其对温度和食物供应的反应。具体而言，我们测试的假设，一个特定的发展阶段的年龄在O。dioica可以从内柱细胞的数量和温度来预测。我们表明，endostyle的增长招募细胞从后尖端到侧臂的器官在一个前-后的方向，并在侧臂endostyle细胞的增加率是温度依赖性的，但对营养摄入无反应。因此，内柱细胞作为一个准确和容易测量的标记来描述发育进程。从概念上讲，这种表征发育进展的方法应该有助于弥合整个生物体衰老过程中的生活史事件和分子机制，通过提供一个共同的实验框架促进跨学科的理解。
In models of growth and life history, and in molecular and cell biology, there is a need for more accurate frames of reference to characterize developmental progression. In Caenorhabditis elegans, complete fate maps of cell lineage provide such a standard of reference. To be more widely applicable, reference frames should be easier to measure while still providing strong predictive capacity. Towards this aim, we have analyzed growth of the endostyle in the appendicularian Oikopleura dioica at the cellular level, and measured its response to temperature and food availability. Specifically, we test the hypothesis that age of a specific developmental stage in O. dioica can be predicted from the number of endostyle cells and temperature. We show that the endostyle grows by recruiting cells from the posterior tip into the lateral arms of the organ in an anterior-posterior orientation and that the rate of increase in lateral arm endostyle cells is temperature-dependent but unresponsive to nutritional intake. Endostyle cells therefore serve as an accurate and easily measured marker to describe developmental progression. Conceptually, such a method of characterizing developmental progression should help bridge life-history events and molecular mechanisms throughout organismal aging, facilitating cross-disciplinary understanding by providing a common experimental framework.