ANALYSIS OF THE AUTOLYSINS OF BACILLUS-SUBTILIS-168 DURING VEGETATIVE GROWTH AND DIFFERENTIATION BY USING RENATURING POLYACRYLAMIDE-GEL ELECTROPHORESIS
ANALYSIS OF THE AUTOLYSINS OF BACILLUS-SUBTILIS-168 DURING VEGETATIVE GROWTH AND DIFFERENTIATION BY USING RENATURING POLYACRYLAMIDE-GEL ELECTROPHORESIS
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DOI：
10.1128/jb.174.2.464-470.1992
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发表时间：
1992-01-01
影响因子：
3.2
通讯作者：
FOSTER, SJ
中科院分区：
文献类型：
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作者：
FOSTER, SJ
采用十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳法，对枯草芽孢杆菌168的自溶素进行了分析。用B在SDS和LiCl提取物和天然细胞壁中检测到90、50、34和30 kDa的四条营养体自溶活性条带（条带A1至A4）。subtilis 168营养细胞壁作为基质掺入凝胶中。四种酶活性表现出不同的底物特异性和不同的化学处理的敏感性。自溶谱不依赖于培养基，在营养生长过程中保持恒定。在孢子形成期间，就在母细胞溶解之前，A4带的活性大大增加。没有发芽相关的变化，在配置文件中观察到，虽然可溶性41-kDa的孢子内相关的皮层裂解酶被发现。通过使用插入失活突变体，带A1和A2被积极确定为以前的特点90-kDa的氨基葡萄糖苷酶和50-kDa的酰胺酶，分别。常见的丝状表型的各种调控突变体不能与特定的变化，在自溶素档案。
The autolysins of Bacillus subtilis 168 were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis with substrate-containing gels. Four bands of vegetative autolytic activity of 90, 50, 34, and 30 kDa (bands A1 to A4) were detected in SDS and LiCl extracts and in native cell walls by using B. subtilis 168 vegetative cell walls as the substrate incorporated in the gel. The four enzyme activities showed different substrate specificities and sensitivities to various chemical treatments. The autolysin profile was not medium dependent and remained constant during vegetative growth. During sporulation, band A4 greatly increased in activity just prior to mother-cell lysis. No germination-associated changes in the profile were observed, although a soluble 41-kDa endospore-associated cortex-lytic enzyme was found. By using insertionally inactivated mutants, bands A1 and A2 were positively identified as the previously characterized 90-kDa glucosaminidase and 50-kDa amidase, respectively. The common filamentous phenotype of various regulatory mutants could not be correlated to specific changes in the autolysin profile.