A versatile method for combining different biopolymers in a core/shell fashion by 3D plotting to achieve mechanically robust constructs
A versatile method for combining different biopolymers in a core/shell fashion by 3D plotting to achieve mechanically robust constructs
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DOI：
10.1088/1758-5090/8/4/045001
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发表时间：
2016-12-01
期刊：
影响因子：
9
通讯作者：
Gelinsky, Michael
中科院分区：
文献类型：
--
作者：
Akkineni, Ashwini Rahul;Ahlfeld, Tilman;Gelinsky, Michael
在过去的几年里，以核/壳（c/s）方式三维挤出两种不同的生物材料已经获得了很大的兴趣，因为它允许制造具有新的和有趣的特性的结构。Wenow证明，将高浓度（16.7wt%）藻酸盐水凝胶作为壳材料与低浓度的软生物聚合物水凝胶作为核相结合，导致机械稳定和坚固的3D支架。海藻酸盐，壳聚糖，结冷胶，明胶和胶原蛋白水凝胶被成功地利用为核心材料-水凝胶，这是太软的开孔结构的3D绘图没有额外的机械支持。分别对c/s支架的形态、力学性能和溶胀行为进行了表征。可以表明，核以及壳部分可以负载生长因子，并且释放取决于核组成和壳厚度。绘图过程和与1 M CaCl 2的交联都没有使蛋白质变性。当核和壳装载有不同的生长因子（分别为VEGF和BMP-2）时，实现了双重释放。最后，活的人类内皮细胞被整合到核心材料中，这表明这种新策略也可以用于生物打印目的。
Three-dimensional extrusion of two different biomaterials in a core/shell (c/s) fashion has gained much interest in the last couple of years as it allows for fabricating constructs with novel and interesting properties. Wenow demonstrate that combining high concentrated (16.7 wt%) alginate hydrogels as shell material with low concentrated, soft biopolymer hydrogels as core leads to mechanically stable and robust 3D scaffolds. Alginate, chitosan, gellan gum, gelatin and collagen hydrogels were utilized successfully as core materials-hydrogels which are too soft for 3D plotting of open-porous structures without an additional mechanical support. The respective c/s scaffolds were characterized concerning their morphology, mechanical properties and swelling behavior. It could be shown that core as well as shell part can be loaded with growth factors and that the release depends on core composition and shell thickness. Neither the plotting process nor the crosslinking with 1M CaCl2 denatured the proteins. When core and shell were loaded with different growth factors (VEGF and BMP-2, respectively) a dual release was achieved. Finally, live human endothelial cells were integrated in the core material, demonstrating that this new strategy can be used for bioprinting purposes as well.