Korean Mistletoe (Viscum album coloratum) Extract Improves Endurance Capacity in Mice by Stimulating Mitochondrial Activity
Korean Mistletoe (Viscum album coloratum) Extract Improves Endurance Capacity in Mice by Stimulating Mitochondrial Activity
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DOI：
10.1089/jmf.2010.1469
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发表时间：
2012-07-01
影响因子：
2.4
通讯作者：
Kim, Jong-Bae
中科院分区：
文献类型：
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作者：
Jung, Hoe-Yune;Lee, An-Na;Kim, Jong-Bae
由于运动对整体健康的有益影响，因此有必要确定能够增强运动效果的口服活性药物，以治疗代谢疾病。已知白藜芦醇（RSV）等天然化合物通过增强线粒体功能来增加耐力。韩国槲寄生（Viscum album coloratum）提取物（KME）具有与RSV相似的特征。在本研究中，我们确定KME是否可以增加线粒体活性并发挥抗疲劳作用。我们发现，KME处理显著提高了L6细胞的线粒体耗氧率（OCR），并增加了C2C12细胞中线粒体功能的两个主要调节因子过氧化物酶体增殖体激活受体γ辅助激活因子(PGC)-1 α和沉默交配型信息调节2同源物1 （SIRT1）的表达。在跑步机测试中，kme治疗小鼠的跑步时间是正常小鼠的2.5倍。此外，kme治疗组疲惫小鼠血浆乳酸水平显著降低。此外，在强迫游泳试验中，KME治疗小鼠的游泳时间延长至疲劳时间达212%。在KME治疗和磷酸盐缓冲盐水治疗的动物之间，肝脏和肾脏组织学相似，表明KME无毒。综上所述，我们的数据表明，KME可能通过激活PGC-1 α和SIRT1来诱导线粒体活性，并提高小鼠的耐力，这强烈表明KME作为一种新型线粒体活化剂具有很大的潜力。
The beneficial effects of exercise on overall health make it desirable to identify the orally active agents that enhance the effects of exercise in an effort to cure metabolic diseases. Natural compounds such as resveratrol (RSV) are known to increase endurance by potentiating mitochondrial function. Korean mistletoe (Viscum album coloratum) extract (KME) has characteristics similar to those of RSV. In the present study, we determined whether KME could increase mitochondrial activity and exert an anti-fatigue effect. We found that KME treatment significantly increased the mitochondrial oxygen consumption rate (OCR) in L6 cells and increased the expression of peroxisome proliferator-activated receptor gamma coactivator (PGC)-1 alpha and silent mating type information regulation 2 homolog 1 (SIRT1), two major regulators of mitochondria function, in C2C12 cells. In the treadmill test, KME-treated mice could run 2.5-times longer than chow-fed control mice. Additionally, plasma lactate levels of exhausted mice were significantly lower in the KME-treated group. In addition, the swimming time to exhaustion of mice treated with KME was prolonged by as much as 212% in the forced-swim test. Liver and kidney histology was similar between the KME-treated and phosphate-buffered saline-treated animals, indicating that KME was nontoxic. Taken together, our data show that KME induces mitochondrial activity, possibly by activating PGC-1 alpha and SIRT1, and improves the endurance of mice, strongly suggesting that KME has great potential as a novel mitochondria-activating agent.