Synthetic Sialyl Compounds as Well as Natural Gangliosides Induce Neuritogenesis in a Mouse Neuroblastoma Cell Line (Neuro2a)
Synthetic Sialyl Compounds as Well as Natural Gangliosides Induce Neuritogenesis in a Mouse Neuroblastoma Cell Line (Neuro2a)
复制标题
合成唾液酸化合物以及天然神经节苷脂诱导小鼠神经母细胞瘤细胞系 (Neuro2a) 中的神经元发生
DOI：
10.1111/j.1471-4159.1988.tb02928.x
复制
发表时间：
1988
影响因子：
4.7
通讯作者：
Y. Nagai
中科院分区：
文献类型：
--
作者：
S. Tsuji;T. Yamashita;Makoto Tanaka;Y. Nagai
含有N‐乙酰神经氨酸（唾液酸）的两亲性化合物[例如，D‐N‐乙酰神经氨酸‐（α2‐1）‐2S, 3R，4E‐2‐N‐四osanoyl鞘碱，唾液酰烷基甘油醚和唾液酰胆固醇]诱导神经母细胞瘤细胞系的神经细胞发生（Neuro2a）。化合物亲水部分中的唾液酸是神经生成所特别需要的。然而，对疏水片段的分子特异性要求很低。关于疏水部分，没有对胆固醇、烷基甘油醚或神经酰胺残基的神经生成活性进行偏好。具有α -酮苷型硅酮键的硅酮化合物比相应的β -异头化合物更有活性。这些唾液酸化合物只诱导了一个神经突的生长，而且是一个长神经突。这种类型的神经生成与能够提高细胞内环状AMP浓度的化合物诱导的神经生成完全不同，后者诱导单个细胞体出现许多神经突。除了这种形态变化外，活性唾液化合物还引起细胞表面碳水化合物组成的变化。化合物处理显著增加花生凝集素结合位点的浓度。
Amphipathic compounds containing N‐acetylneuraminic acid (sialic acid) [for example, D‐N‐acetylneuraminyl‐(α2‐1)‐2S, 3R,4E‐2‐N‐tetracosanoyl sphingenine, sialyl alkyl glycerol ethers, and sialyl cholesterols] induced neuritogenesis in a neuroblastoma cell line (Neuro2a). The sialic acid in the hydrophilic moiety of the compounds is specifically required for neuritogenesis. The requirement for molecular specificity of the hydrophobic moiety, however, is rather low. Regarding the hydrophobic moiety, no preference for cholesterol, alkyl glycerol ether, or ceramide residues was observed as to their neuritogenic activity. Sialyl compounds with α‐ketosidic sialyl linkages were more active than the corresponding β‐anomers. These sialyl compounds induced the growth of only one neurite, but a long one, from the cell body. This type of neuritogenesis is completely different from that induced by compounds capable of elevating the concentration of intracellular cyclic AMP, which induced the appearance of many neurites from a single cell body. Besides this morphological change, the active sialyl compounds also caused a change in the carbohydrate composition of the cell surface. Sialyl compound treatment drastically increased the concentration of peanut agglutinin binding sites.
神经节苷脂对蛋白激酶 C 活性的调节。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kreutter,D;Kim,JY;Goldenring,JR;Rasmussen,H;Ukomadu,C;DeLorenzo,RJ;Yu,RK
通讯作者：
Yu,RK