Dual-energy computed tomography imaging of atherosclerotic plaques in a mouse model using a liposomal-iodine nanoparticle contrast agent.
Dual-energy computed tomography imaging of atherosclerotic plaques in a mouse model using a liposomal-iodine nanoparticle contrast agent.
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DOI：
10.1161/circimaging.112.000119
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发表时间：
2013-03-01
期刊：
影响因子：
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通讯作者：
Annapragada A
中科院分区：
文献类型：
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作者：
Bhavane R;Badea C;Ghaghada KB;Clark D;Vela D;Moturu A;Annapragada A;Johnson GA;Willerson JT;Annapragada A
巨噬细胞在动脉粥样硬化斑块中的积累早已被认识到。为了开发一种检测易损斑块的显像剂，我们在小鼠模型中评估了脂质体-碘纳米颗粒造影剂用于富含巨噬细胞的动脉粥样硬化斑块的计算机断层扫描（CT）成像的可行性。制备了不同粒径和聚乙二醇包覆的脂质体-碘制剂，并证明其能稳定地包覆碘化合物。在RAW264.7巨噬细胞系中使用光学和CT成像进行体外摄取研究，确定了促进最大摄取的配方。使用该配方对载脂蛋白E缺陷（ApoE−/−）小鼠（n=8）和对照C57BL/6小鼠（n=6）进行双能CT成像，然后对双能图像进行光谱分解，使定位在斑块中的脂质体成像。成像细胞术证实斑块中存在脂质体，并与斑块中一小部分（约2%）巨噬细胞共定位。结果证明了脂质体碘纳米颗粒造影剂和双能CT成像富含巨噬细胞的动脉粥样硬化斑块的可行性。
The accumulation of macrophages in inflamed atherosclerotic plaques has been long recognized. In an attempt to develop an imaging agent for detection of vulnerable plaques, we evaluated the feasibility of a liposomal-iodine nanoparticle contrast agent for computed tomography (CT) imaging of macrophage-rich atherosclerotic plaques in a mouse model. Liposomal-iodine formulations varying in particle size and polyethylene glycol coating were fabricated, and shown to stably encapsulate the iodine compound. In vitro uptake studies using optical and CT imaging in the RAW264.7 macrophage cell line identified the formulation that promoted maximal uptake. Dual-energy CT imaging using this formulation in Apolipoprotein E deficient (ApoE−/−) mice (n=8) and control C57BL/6 mice (n=6) followed by spectral decomposition of the dual-energy images enabled imaging of the liposomes localized in the plaque. Imaging cytometry confirmed the presence of liposomes in the plaque and their co-localization with a small fraction (~2%) of the macrophages in the plaque. The results demonstrate the feasibility of imaging macrophage-rich atherosclerotic plaques using a liposomal-iodine nanoparticle contrast agent and dual-energy CT.