A CONSENSUS REPEAT SEQUENCE FROM THE HUMAN INSULIN GENE LINKED POLYMORPHIC REGION ADOPTS MULTIPLE QUADRIPLEX DNA STRUCTURES INVITRO
A CONSENSUS REPEAT SEQUENCE FROM THE HUMAN INSULIN GENE LINKED POLYMORPHIC REGION ADOPTS MULTIPLE QUADRIPLEX DNA STRUCTURES INVITRO
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DOI：
10.1016/0014-5793(92)80214-2
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发表时间：
1992-04-13
期刊：
影响因子：
3.5
通讯作者：
DOCHERTY, K
中科院分区：
文献类型：
--
作者：
HAMMONDKOSACK, MCU;DOCHERTY, K
由14个碱基对（bp）共有序列ACAGGGGT（G/C）T/C）GGGG的重复组成的高变区位于人胰岛素基因上游363 bp处。如果该寡核苷酸产生多态性，则重复次数不同，因此该区域通常称为胰岛素基因连锁多态性区域（ILPR）。在这里，我们提出的证据，基于在非变性聚丙烯酰胺凝胶中的流动性的两个大小不同的寡核苷酸含有ILPR的共识序列，该序列可以在体外采用一些四链体DNA结构。
A hypervariable region consisting of repeats of a 14 base pair (bp) consensus sequence ACAGGGGT(G/C))T/C)GGGG is located 363 bp upstream of the human insulin gene. Different repeat numbers if this oligonucleotide give rise to a polymorphism, and so this region is commonly known as the insulin gene linked polymorphic region (ILPR). Here we present evidence, based on the mobility in non-denaturing polyacrylamide gels of two dissimilarly sized oligonucleotides containing the ILPR consensus sequence, that this sequence can adopt a number of quadriplex DNA structures in vitro.