Isolation and identification of bacteria and fungi from cinematographic films
Isolation and identification of bacteria and fungi from cinematographic films
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DOI：
10.1016/j.ibiod.2005.05.004
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发表时间：
2005-07-01
影响因子：
4.8
通讯作者：
Platas, G
中科院分区：
文献类型：
--
作者：
Abrusci, C;Martin-González, A;Platas, G
马德里、巴塞罗那和大加那利岛西班牙档案馆的黑白电影胶片样本中存在的细菌和真菌被分离和鉴定。所有研究的样品均被污染。分离出十四株细菌。通过形态学和生化生理学方法进行初步表征，根据细菌性质使用不同的商业测定法。五种葡萄球菌，即。 S. epidermidis、S. hominis、S. lentus、S. haemolyticus 和 S. lugdunensis 以及五种芽孢杆菌，即：鉴定出解淀粉芽孢杆菌、枯草芽孢杆菌、巨大芽孢杆菌、皮基诺氏芽孢杆菌和短小芽孢杆菌，以及少动鞘氨醇单胞菌、克里斯蒂娜库库里亚和溶血性巴斯德氏菌。通过微卫星引发的 PCR 和 rDNA 测序分离并鉴定了 17 株丝状真菌和一种酵母菌（Cryptococcus albidus）。薄膜中存在的真菌菌株由四种曲霉菌株组成，即： A. ustus、A. nidulans var.nidulans、A. versicolor、七种青霉菌株，以及链格孢、枝孢枝孢、总状毛霉、球茎点霉和长枝木霉。与真菌分离株相比，只有七种细菌菌株能够降解明胶（照相乳液中的粘合剂），所有真菌分离株都液化明胶，但酵母浅白隐球菌除外。电影胶片中发现的大多数微生物都表现出对不利环境条件的抵抗力。 (c) 2005 Elsevier Ltd. 保留所有权利。
Bacteria and fungi present in black and white cinematographic film samples from Spanish archives in Madrid, Barcelona and Gran Canaria were isolated and identified. All samples studied were contaminated. Fourteen strains of bacteria were isolated. Preliminary characterization was carried out by morphological and biochemical-physiological methods, using different commercial assays depending on the bacterial nature. Five species of Staphylococcus, viz. S. epidermidis, S. hominis, S. lentus, S. haemolyticus and S. lugdunensis, and five species of Bacillus, viz. B. amyloliquefaciens, B. subtilis, B. megaterium, B. pichinotyi and B. pumilus, were identified, together with Sphingomonas paucimobilis, Kocuria kristinae and Pasteurella haemolytica. Seventeen strains of filamentous fungi and one yeast, Cryptococcus albidus, were isolated and identified by microsatellite-primed PCR and rDNA sequencing. The fungal strains present in the films consisted of four strains of Aspergillus viz. A. ustus, A. nidulans var.nidulans, A. versicolor, seven Penicillium chrysogenum strains, as well as, Alternaria alternata, Cladosporium cladosporioides, Mucor racemosus, Phoma glomerata and Trichoderma longibrachiatum. Only seven bacterial strains were able to degrade gelatin (binder in photographic emulsions), in contrast to the fungal isolates, all of which liquefied gelatin, with the exception of the yeast Cryptococcus albidus. Most of the microorganisms that were found colonizing the cinematographic films show resistance to adverse environmental conditions. (c) 2005 Elsevier Ltd. All rights reserved.