Interest of bacterial pangenome analyses in clinical microbiology
Interest of bacterial pangenome analyses in clinical microbiology
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DOI：
10.1016/j.micpath.2020.104275
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发表时间：
2020-12-01
影响因子：
3.8
通讯作者：
Fournier, Pierre-Edouard
中科院分区：
文献类型：
--
作者：
Anani, Hussein;Zgheib, Rita;Fournier, Pierre-Edouard
由于基因组测序技术的进步和成本的降低，目前公共数据库中有超过250，000个细菌基因组，涵盖了大多数（如果不是全部的话）与人类相关的致病或非致病微生物的主要系统发育组。此外，对于其中许多物种，可以获得某一物种几个菌株的序列，从而能够评估其遗传多样性并研究其进化。此外，细菌全基因组测序的显著成本降低以及可用细菌基因组数量的快速增加促进了泛基因组学软件工具的开发。细菌泛基因组的研究在临床微生物学中有许多应用。它可以揭示细菌的致病潜力和抵抗抗菌剂的能力，以及鉴定特定序列和预测抗原表位，从而允许设计分子或血清学检测和疫苗。细菌泛基因组是了解人类细菌历史并将这些发现与临床微生物学实验室的诊断相关联以优化患者管理的有力方法。
Thanks to the progress and decreasing costs in genome sequencing technologies, more than 250,000 bacterial genomes are currently available in public databases, covering most, if not all, of the major human-associated phylogenetic groups of these microorganisms, pathogenic or not. In addition, for many of them, sequences from several strains of a given species are available, thus enabling to evaluate their genetic diversity and study their evolution. In addition, the significant cost reduction of bacterial whole genome sequencing as well as the rapid increase in the number of available bacterial genomes have prompted the development of pangenomic software tools. The study of bacterial pangenome has many applications in clinical microbiology. It can unveil the pathogenic potential and ability of bacteria to resist antimicrobials as well identify specific sequences and predict antigenic epitopes that allow molecular or serologic assays and vaccines to be designed. Bacterial pangenome constitutes a powerful method for understanding the history of human bacteria and relating these findings to diagnosis in clinical microbiology laboratories in order to optimize patient management.