The influence of structural design of PLGA/collagen hybrid scaffolds in cartilage tissue engineering
The influence of structural design of PLGA/collagen hybrid scaffolds in cartilage tissue engineering
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PLGA/胶原杂化支架结构设计对软骨组织工程的影响
DOI：
10.1016/j.biomaterials.2009.11.070
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发表时间：
2010-03-01
期刊：
影响因子：
14
通讯作者：
Chen, Guoping
中科院分区：
文献类型：
--
作者：
Dai, Wenda;Kawazoe, Naoki;Chen, Guoping
三维可生物降解多孔支架在关节软骨组织工程中具有重要作用。我们开发了3D支架的混合结构，结合了天然I型胶原蛋白和合成PLGA编织网的优点。机械强度高的PLGA网充当骨架，而胶原微海绵促进细胞接种和组织形成。将支架分为3组：（1）THIN：PLGA网间隙形成的胶原微海绵;（2）SEMI：PLGA网一侧形成的胶原微海绵;（3）SANDWICH：PLGA网两侧形成的胶原海绵。将牛软骨细胞在这些支架中培养，并皮下移植到裸鼠体内2、4和8周。所有三组移植物均显示出均匀的细胞分布、天然软骨细胞形态和丰富的软骨ECM沉积。SEMI组和SANDWICH组中每个DNA的GAG产量以及11型胶原和聚集蛋白聚糖mRNA的表达均远高于THIN组。与天然软骨相比，工程软骨的机械强度在SEMI和SANDWICH中分别达到54.8%和49.3%的杨氏模量和68.8%和62.7%的刚度。该支架可用于厚度可调的关节软骨组织工程。杂化结构的设计为制备3D多孔支架提供了一种策略。(C)2009爱思唯尔有限公司保留所有权利。
3D biodegradable porous scaffold plays a very important role in articular cartilage tissue engineering. We developed hybrid structures of 3D scaffolds that combined the advantages of natural type I collagen and synthetic PLGA knitted mesh. The mechanically strong PLGA mesh served as a skeleton while the collagen microsponges facilitated cell seeding and tissue formation. The scaffolds were divided into 3 groups: (1) THIN: collagen microsponge formed in interstices of PLGA mesh; (2) SEMI: collagen microsponge formed on one side of PLGA mesh; (3) SANDWICH: collagen sponge formed on both sides of PLGA mesh. Bovine chondrocytes were cultured in these scaffolds and transplanted subcutaneously into nude mice for 2, 4, and 8 weeks. All three groups of transplants showed homogeneous cell distribution, natural chondrocyte morphology, and abundant cartilaginous ECM deposition. Production of GAGs per DNA and the expression of type 11 collagen and aggrecan mRNA were much higher in the SEMI and SANDWICH groups than in the THIN group. When compared to native articular cartilage, the mechanical strength of the engineered cartilage reached 54.8%, 49.3% in Young's modulus and 68.8%, 62.7% in stiffness, respectively, in SEMI and SANDWICH. These scaffolds could be used for the tissue engineering of articular cartilage with adjustable thickness. The design of the hybrid structures provides a strategy for the preparation of 3D porous scaffolds. (C) 2009 Elsevier Ltd. All rights reserved.