Quantitative analysis of wet-heat inactivation in bovine spongiform encephalopathy
Quantitative analysis of wet-heat inactivation in bovine spongiform encephalopathy
复制标题
牛海绵状脑病湿热灭活的定量分析
DOI：
10.1016/j.bbrc.2013.01.081
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Mohri S.
中科院分区：
文献类型：
--
作者：
Matsuura Y;Ishikawa Y;Bo X;Murayama Y;Yokoyama T;Somerville RA;Kitamoto T;Mohri S.
The bovine spongiform encephalopathy (BSE) agent is resistant to conventional microbial inactivation procedures and thus threatens the safety of cattle products and by-products. To obtain information necessary to assess BSE inactivation, we performed quantitative analysis of wet-heat inactivation of infectivity in BSE-infected cattle spinal cords. Using a highly sensitive bioassay, we found that infectivity in BSE cattle macerates fell with increase in temperatures from 133°C to 150°C and was not detected in the samples subjected to temperatures above 155°C. In dry cattle tissues, infectivity was detected even at 170°C. Thus, BSE infectivity reduces with increase in wet-heat temperatures but is less affected when tissues are dehydrated prior to the wet-heat treatment. The results of the quantitative protein misfolding cyclic amplification assay also demonstrated that the level of the protease-resistant prion protein fell below the bioassay detection limit by wet-heat at 155°C and higher and could help assess BSE inactivation. Our results show that BSE infectivity is strongly resistant to wet-heat inactivation and that it is necessary to pay attention to BSE decontamination in recycled cattle by-products.
