CATABOLISM OF PHENYLPROPIONIC ACID AND ITS 3-HYDROXY DERIVATIVE BY ESCHERICHIA-COLI
CATABOLISM OF PHENYLPROPIONIC ACID AND ITS 3-HYDROXY DERIVATIVE BY ESCHERICHIA-COLI
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DOI：
10.1128/jb.155.1.113-121.1983
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发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
CHAPMAN, PJ
中科院分区：
文献类型：
--
作者：
BURLINGAME, R;CHAPMAN, PJ
一些实验室菌株和临床分离株利用几种芳香酸作为唯一的C来源进行生长。E.ColiK-12利用分离反应将3-苯丙酸和3-(3-羟基苯基)丙酸转化为3-(2，3-二羟基苯基)丙酸，苯核裂解后生成丁二酸酯、丙酮酸和乙醛。酶分析和呼吸测量表明，该分支途径的所有酶都是可诱导的，将2个初始生长底物转化为3-(2，3-二羟基苯基)丙酸酯所需的酶的合成受到单独的控制。大肠杆菌K-12也以3-羟基肉桂酸为唯一碳源生长，研究了细胞氧化肉桂酸、3-苯丙酸及羟化衍生物的能力。从酶促反应混合物中分离得到4-羟基-2-酮戊酸内酯，并对其光学活性等性质进行了研究。
A number of laboratory strains and clinical isolates of E. coli utilized several aromatic acids as sole sources of C for growth. E. coli K-12 used separate reactions to convert 3-phenylpropionic and 3-(3-hydroxyphenyl)propionic acids into 3-(2,3-dihydroxphenyl)propionic acid which, after meta-fission of the benzene nucleus, gave succinate, pyruvate and acetaldehyde as products. Enzyme assays and respirometry showed that all enzymes of this branched pathway were inducible and that syntheses of enzymes required to convert the 2 initial growth substrates into 3-(2,3-dihydroxyphenyl)propionate are under separate control. E. coli K-12 also grew with 3-hydroxycinnamic acid as sole source of C; the ability of cells to oxidize cinnamic and 3-phenylpropionic acids and hydroxylated derivatives was investigated. The lactone of 4-hydroxy-2-ketovaleric acid was isolated from enzymatic reaction mixtures and its properties, including optical activity, were rcorded.