Glucose stabilizes collagen sterilized with gamma irradiation.
Glucose stabilizes collagen sterilized with gamma irradiation.
复制标题
葡萄糖可稳定经伽马射线照射灭菌的胶原蛋白。
DOI：
10.1002/jbm.a.20018
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Dunn,MichaelG
中科院分区：
文献类型：
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作者：
Ohan,MarkP;Dunn,MichaelG
伽马辐照灭菌（γ辐照）使胶原蛋白碎片和变性，从而显著降低关键物理性能。我们的目标是通过添加葡萄糖来保持γ辐照胶原的强度和稳定性，理论上，葡萄糖可以在暴露于γ辐照期间引发胶原中的交联形成。用2.5 Mrad的标准剂量对用和不用葡萄糖制备的胶原膜进行γ辐照。交联和变性的相对量基于水合、热变性或在酶（胶原酶和胰蛋白酶）中孵育后膜的溶解度和机械性质来近似。暴露于γ辐照后，与对照膜相比，含有葡萄糖的胶原膜具有显著更高的机械性能、更大的酶降解抗性和降低的溶解度。用第二组膜重复整个实验，所述第二组膜首先暴露于紫外线照射（254 nm）以提供更高的初始强度，然后进行γ照射。同样，与对照相比，含有葡萄糖的膜具有显著更大的机械性能和对酶降解的抗性。凝胶电泳显示葡萄糖不能阻止肽片段化;因此，葡萄糖掺入膜的较高强度和稳定性可能是由于葡萄糖衍生的交联。本研究的结果表明，葡萄糖可能是一种有用的添加剂，用于稳定经γ辐照灭菌的胶原材料或组织。© 2003 Wiley Periodicals，Inc. J Biomed Mater Res 67A：1188-1195，2003
Gamma irradiation sterilization (γ‐irradiation) fragments and denatures collagen, drastically decreasing critical physical properties. Our goal was to maintain strength and stability of γ‐irradiated collagen by adding glucose, which in theory can initiate crosslink formation in collagen during exposure to γ‐irradiation. Collagen films prepared with and without glucose were γ‐irradiated with a standard dose of 2.5 Mrad. Relative amounts of crosslinking and denaturation were approximated based on solubility and the mechanical properties of the films after hydration, heat denaturation, or incubation in enzymes (collagenase and trypsin). After exposure to γ‐irradiation, collagen films containing glucose had significantly higher mechanical properties, greater resistance to enzymatic degradation, and decreased solubility compared with control films. The entire experiment was repeated with a second set of films that were exposed first to ultraviolet irradiation (254 nm) to provide higher initial strength and then γ‐irradiated. Again, films containing glucose had significantly greater mechanical properties and resistance to enzymatic degradation compared with controls. Gel electrophoresis showed that glucose did not prevent peptide fragmentation; therefore, the higher strength and stability in glucose‐incorporated films may be due to glucose‐derived crosslinks. The results of this study suggest that glucose may be a useful additive to stabilize collagenous materials or tissues sterilized by γ‐irradiation. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 1188–1195, 2003