Quantitation of hepatitis B surface antigen by an automated chemiluminescent microparticle immunoassay
Quantitation of hepatitis B surface antigen by an automated chemiluminescent microparticle immunoassay
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DOI：
10.1016/j.jviromet.2003.10.002
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发表时间：
2004-02-01
影响因子：
3.1
通讯作者：
Mushahwar, IK
中科院分区：
文献类型：
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作者：
Deguchi, M;Yamashita, N;Mushahwar, IK
采用全自动荧光微粒子免疫测定法（Architect HBsAg QT）检测和定量B型肝炎表面抗原（HBsAg）。该检测试剂盒每小时能够处理多达800个HBsAg检测。使用先前生成的Architect HBsAg校准曲线测定HBsAg浓度。发现Architect HBsAg QT灵敏度约为0.2 ng/ml，与其他已知和市售酶免疫测定法和/或荧光免疫测定法相当或上级。本文对43例慢性B型肝炎携带者733份血清中HBsAg、HBeAg、HBV-DNA及HBV-DNA聚合酶进行了定量研究。Architect HBsAg QT检测HBeAg阳性者血清HBsAg水平高于抗-HBe阳性者，且与血清HBV-DNA和HBV-DNA聚合酶水平相关。(C)2003 Elsevier B. V.保留所有权利。
A fully automated chemiluminescent microparticle immunoassay (Architect HBsAg QT) was used for the detection and quantitation of hepatitis B surface antigen (HBsAg). The assay is capable of processing up to 800 HBsAg tests per hour. The concentration of HBsAg is determined by utilizing a previously generated Architect HBsAg calibration curve. Architect HBsAg QT sensitivity was found to be around 0.2 ng/ml which is equivalent or superior to other known and commercially available enzyme immunoassays and/or chemiluminescent immunoassays. We performed a quantitative study of HBsAg, HBeAg, HBV-DNA and HBV-DNA polymerase in over 733 sera obtained from 43 chronic hepatitis B carriers. Serum HBsAg levels detected by Architect HBsAg QT were found to be higher in HBeAg-positive than in anti-HBe-positive HBV chronic carriers and correlated with the level of serum HBV-DNA and HBV-DNA polymerase. (C) 2003 Elsevier B.V. All rights reserved.