CHARACTERIZATION OF AN AROMATIC AMINO ACID-DEPENDENT LISTERIA-MONOCYTOGENES MUTANT - ATTENUATION, PERSISTENCE, AND ABILITY TO INDUCE PROTECTIVE IMMUNITY IN MICE
CHARACTERIZATION OF AN AROMATIC AMINO ACID-DEPENDENT LISTERIA-MONOCYTOGENES MUTANT - ATTENUATION, PERSISTENCE, AND ABILITY TO INDUCE PROTECTIVE IMMUNITY IN MICE
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DOI：
10.1128/iai.61.5.2245-2248.1993
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发表时间：
1993-05-01
影响因子：
3.1
通讯作者：
ROBERTS, IS
中科院分区：
文献类型：
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作者：
ALEXANDER, JE;ANDREW, PW;ROBERTS, IS
单核细胞增多性李斯特氏菌的一个转座子插入突变体被证明缺乏预苯酸脱水酶，该酶在苯丙氨酸生物合成途径中的作用较晚。这个突变体降低了小鼠的毒力。突变菌株和亲本菌株在感染小鼠的组织中持续存在的程度相同，并引起类似程度的脾肿大。接种该突变体的小鼠对随后的毒力单核细胞增多性李斯特菌攻击有显著的保护作用。
A transposon insertion mutant of Listeria monocytogenes was shown to be deficient in prephenate dehydratase, an enzyme acting late in the pathway for biosynthesis of phenylalanine. This mutant had reduced virulence in mice. The mutant and parent strains persisted to the same extent in the tissues of infected mice and elicited similar degrees of splenomegaly. Mice vaccinated with the mutant were protected significantly from subsequent challenge with virulent L. monocytogenes.