Distribution of RGS4 mRNA in mouse brain shown by in situ hybridization.
Distribution of RGS4 mRNA in mouse brain shown by in situ hybridization.
复制标题
原位杂交显示小鼠大脑中 RGS4 mRNA 的分布。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
K. Kiuchi
中科院分区：
文献类型：
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作者：
Satoshi Nomoto;Kayo Adachi;Li;Y. Hirata;Sachiko Muraguchi;K. Kiuchi
RGS蛋白（regulators of G protein signaling）是一组新近发现的负调节G蛋白的蛋白质。一些，包括RGS4，已被证明作为GTP酶激活蛋白的几个成员的Gi α亚基和Gq α亚基。然而，由RGS4调节的G蛋白偶联受体的信号转导途径还不清楚。为了研究RGS4蛋白在小鼠脑中的作用，我们分离了小鼠RGS4 cDNA。小鼠RGS4在酵母中的表达刺激了对交配信息素的适应，这表明它负调节G蛋白介导的信号通路。各种小鼠组织的北方印迹分析显示，RGS4在脑中以高水平表达，在心脏中以中等低水平表达，在肺、肝和骨骼肌中以非常低的水平表达。小鼠脑组织原位杂交显示RGS4 mRNA主要分布于大脑皮质、海马、嗅前核、梨状皮质、嗅结节、尾壳核、杏仁核、Calleja岛、黑质、杏仁核、小脑颗粒层、小脑中脚和面周带。这些结果表明，RGS4在大脑中的各种类型的细胞中表达，并可能调节多种G蛋白介导的信号通路。
RGS proteins (regulators of G protein signaling) are a newly identified group of proteins that negatively regulate G proteins. Some, including RGS4, have been shown to act as GTPase-activating proteins for several members of the Gi alpha subunits and the Gq alpha subunit. However, the signaling pathways of G protein-coupled receptors regulated by RGS4 are not well understood. We isolated the mouse RGS4 cDNA to investigate the roles of RGS4 protein in mouse brain. Expression of mouse RGS4 in yeast stimulated adaptation to mating pheromone, suggesting that it negatively regulates that G protein-mediated signaling pathway. Northern blot analysis of various mouse tissues revealed that RGS4 is expressed at high levels in brain, moderately low levels in heart, and very low levels in lung, liver, and skeletal muscle. In situ hybridization of mouse brain showed RGS4 mRNA mainly in the cerebral cortex, hippocampus, anterior olfactory nucleus, piriform cortex, olfactory tubercle, caudate-putamen, nucleus accumbens, islands of Calleja, substantia nigra, amygdala, the granular layer of cerebellum, middle cerebellar peduncle, and perifacial zone. These results suggest that RGS4 is expressed in various types of cells in the brain and may regulate multiple G protein-mediated signaling pathways.