MEASUREMENT OF THE TESTOSTERONE BINDING PARAMETERS FOR BOTH TESTOSTERONE-ESTRADIOL BINDING GLOBULIN AND ALBUMIN IN INDIVIDUAL SERUM SAMPLES
MEASUREMENT OF THE TESTOSTERONE BINDING PARAMETERS FOR BOTH TESTOSTERONE-ESTRADIOL BINDING GLOBULIN AND ALBUMIN IN INDIVIDUAL SERUM SAMPLES
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DOI：
10.1016/0039-128x(79)90090-4
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发表时间：
1979-01-01
期刊：
影响因子：
2.7
通讯作者：
DUNN, JF
中科院分区：
文献类型：
--
作者：
NISULA, BC;DUNN, JF
本报告描述了一种固相方法，用于表征睾酮[T]与白蛋白和睾酮-雌二醇结合球蛋白(TeBG)的结合。TeBG从血清样本中被吸附到刀豆蛋白A的固相基质上，该基质共价连接到4B琼脂糖凝胶上。然后在存在和不存在内源性血清白蛋白的情况下检测T的结合。对所得Scatchard图的分析允许确定TeBG结合容量、TeBG结合常数[Ka]和白蛋白结合参数，该参数相当于其与T的亲和力和结合能力的乘积。男性的TeBG容量低于女性(18.4±-)。5.8比33.1+-。1.19.2海里，P<；0.01)。男性的KA更大(1.59。+-)。0.35比1.19+-0.32次。109m-1,109m-1，P<；0.01)。白蛋白结合参数(43.8.+-)无差异。18.3比46.6.+-15.5，NS[不显著])。然后可以使用这些参数来计算循环T在白蛋白结合组分、TeBG结合组分和未结合组分中的分布。
This report describes a solid phase method for the characterization of testosterone [T] binding to both albumin and testosterone-estradiol binding globulin (TeBG). TeBG is adsorbed from serum samples onto a solid phase matrix of concanavalin A covalently linked to 4 B Sepharose. The binding of T is then examined both in the presence and absence of the endogenous serum albumin. Analysis of the resulting Scatchard plots permits determination of the TeBG binding capacity, TeBG association constant [Ka] and a parameter of albumin binding equivalent to the product of its affinity and capacity for binding T. The TeBG capacity was lower in men than in women (18.4 .+-. 5.8 vs. 33.1 .+-. 1.19.2 nM, P < 0.01). The Ka was greater in men (1.59 .+-. 0.35 vs. 1.19 .+-. 0.32 .times. 109M-1, 109M-1, P < 0.01). There was no difference in the albumin binding parameter (43.8 .+-. 18.3 vs. 46.6 .+-. 15.5, NS [non-significant]). These parameters can then be used to calculate the distribution of the circulating T into albumin bound, TeBG bound and unbound fractions.