Cell-free biosynthesis of somatostatin precursors: Evidence for multiple forms of preprosomatostatin.
Cell-free biosynthesis of somatostatin precursors: Evidence for multiple forms of preprosomatostatin.
复制标题
生长抑素前体的无细胞生物合成：多种形式的前生长抑素的证据。
DOI：
10.1073/pnas.79.12.3729
复制
发表时间：
1982
影响因子：
11.1
通讯作者：
Shields,D
中科院分区：
文献类型：
--
作者：
Warren,TG;Shields,D
[Shields， D. (1980)] J. Biol。从Langerhans胰岛分离的mRNA分别编码表观分子量为18,000和19,000的两个预生长抑素分子。本研究的证据表明，在两种不同的无细胞蛋白合成系统中，胰岛mRNA的体外翻译指导了多达九种不同形式的生长抑素免疫反应多肽的合成。用n -甲酰基-[35S]Met-tRNAMetf标记氨基末端可以证明，促生长抑素前分子的多样性是来自不同种类mRNA的翻译起始的结果。从不同动物个体中分离的胰岛mRNA的翻译表明，在无细胞产物中存在所有的预生长抑素多肽，这意味着多种形式不是由于野生群体的遗传变异。根据其表观分子量和明显不同的等电点，不同的促生长抑素前分子可分为两大科。这些结果表明，生长素前促生长抑素是由不同的mRNA物种编码的，并且与生长抑素多基因家族的存在相一致。
It has been demonstrated [Shields, D. (1980) J. Biol. Chem. 255, 11625-11628] that mRNA isolated from the islets of Langerhans codes for two preposomatostatin molecules of apparent molecular weights 18,000 and 19,000, respectively. Here evidence is presented that in vitro translation of pancreatic islet mRNA in two different cell-free protein-synthesizing systems directs the synthesis of up to nine distinct forms of somatostatin-immunoreactive polypeptides. The multiplicity of the preprosomatostatin molecules was the result of initiation of translation from separate species of mRNA as demonstrated by amino-terminal labeling with N-formyl-[35S]Met-tRNAMetf. Translation of islet mRNA isolated from different individual animals showed that all of the preposomatostatin polypeptides were present amongst the cell-free products, which implies that the multiple forms were not due to genetic variation in the wild population. Based on their apparent molecular weights and distinctly different isoelectric points, the different preprosomatostatin molecules could be classified into two major families. These results suggest that the anglerfish preprosomatostatins are encoded by separate mRNA species and are consistent with the existence of a multigene family for somatostatin.