Substrate-independent transduction of chromophore-free organic and biomolecular transformations into color
Substrate-independent transduction of chromophore-free organic and biomolecular transformations into color
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DOI：
10.1002/chem.200500977
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发表时间：
2006-04-03
影响因子：
4.3
通讯作者：
Matile, S
中科院分区：
文献类型：
--
作者：
Dash, G;Matile, S
引入了合成多功能孔作为化学反应的与底物无关的光信号传感器的概念，重点是与生物分子转化中底物特异性信号产生的结合。与传统生物传感器中已知的一般电化学转导和免疫传感中已知的分析物特异性生物分子识别（而不是转化）的一般光学转导进行比较，揭示了该概念的基本性质以及与现有方法的有吸引力的互补性。转移酶、水解酶、裂解酶甚至异构酶的例子表明，具有合成多功能孔的光转导通常远远超出电化学转导的底物特异性信号发生器，即氧化还原酶，并且绝对没有问题。在某种程度上，最近的突破被用来强调合成多功能孔作为生物分子转化的光学传感器在实际传感和筛选应用方面的卓越前景。
The concept of synthetic multifunctional pores as substrate-independent optical signal transducers of chemical reactions is introduced with emphasis on the combination with substrate-specific signal generation in biomolecular transformations. Comparison with the general electrochemical transduction, known from conventional biosensors, and the general optical transduction of analyte-specific biomolecular recognition (rather than transformation), known from immunosensing, reveals the fundamental nature of the concept as well as an attractive complementarity to existing methods. Examples with transferases, hydrolases, lyases, and even an isomerase demonstrate that optical transduction with synthetic multifunctional pores is general far beyond the substrate-specific signal generators of electrochemical transduction, that is, the oxidoreductases, and absolutely unproblematic. In part very recent breakthroughs are used to highlight the remarkable promise of synthetic multifunctional pores as optical transducers of biomolecular transformation with regard to practical sensing and screening applications.