Quantitative tumor depth determination using dual wavelength excitation fluorescence.
Quantitative tumor depth determination using dual wavelength excitation fluorescence.
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DOI：
10.1364/boe.468059
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发表时间：
2022-08
影响因子：
3.4
通讯作者：
Christine M. O’Brien;Kevin Bishop;Haini Zhang;Xiao Xu;L. Shmuylovich;Elizabeth Conley;Karen Nwosu;K. Duncan;Suman B Mondal;G. Sudlow;S. Achilefu
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文献类型：
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作者：
Christine M. O’Brien;Kevin Bishop;Haini Zhang;Xiao Xu;L. Shmuylovich;Elizabeth Conley;Karen Nwosu;K. Duncan;Suman B Mondal;G. Sudlow;S. Achilefu
使用荧光引导手术方法量化实体瘤边缘是一项挑战，特别是在使用近红外（NIR）波长时，由于穿透深度增加。开发了一种近红外双波长激发荧光（DWEF）方法，该方法利用组织中光的波长依赖性衰减来确定荧光团深度。构建了便携式双波长激发荧光成像系统，并在组织模拟模型、鸡组织和体内小鼠乳腺癌模型中与NIR肿瘤靶向荧光团并行测试。该系统在所有的实验中表现出很高的精度。这种方法的低成本和简单性使其成为临床应用的理想选择。
Quantifying solid tumor margins with fluorescence-guided surgery approaches is a challenge, particularly when using near infrared (NIR) wavelengths due to increased penetration depths. An NIR dual wavelength excitation fluorescence (DWEF) approach was developed that capitalizes on the wavelength-dependent attenuation of light in tissue to determine fluorophore depth. A portable dual wavelength excitation fluorescence imaging system was built and tested in parallel with an NIR tumor-targeting fluorophore in tissue mimicking phantoms, chicken tissue, and in vivo mouse models of breast cancer. The system showed high accuracy in all experiments. The low cost and simplicity of this approach make it ideal for clinical use.