SEQUENCE IDENTIFICATION OF 2,375 HUMAN BRAIN GENES
SEQUENCE IDENTIFICATION OF 2,375 HUMAN BRAIN GENES
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DOI：
10.1038/355632a0
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发表时间：
1992-02-13
期刊：
影响因子：
64.8
通讯作者：
VENTER, JC
中科院分区：
文献类型：
--
作者：
ADAMS, MD;DUBNICK, M;VENTER, JC
我们最近描述了一种通过部分DNA测序来快速表征表达基因的新方法，以产生“表达序列标签”1。在一组600个人脑互补DNA克隆中，348个是信息丰富的核编码信使rna。目前，我们已经对从四个人脑cDNA文库中分离出来的2672个新的、独立的cDNA克隆进行了部分测序，生成了2375个核编码基因的表达序列标签。这些序列，加上我们之前研究的348个大脑表达序列标签，组成了2500多个新的人类基因和870,769个碱基对的DNA序列。这些数据代表了DNA测序鉴定的人类基因数量的大约两倍，可能代表了人类基因组中多达5%的基因。
WE recently described a new approach for the rapid characterization of expressed genes by partial DNA sequencing to generate 'expressed sequence tags' 1. From a set of 600 human brain complementary DNA clones, 348 were informative nuclear-encoded messenger RNAs. We have now partially sequenced 2,672 new, independent cDNA clones isolated from four human brain cDNA libraries to generate 2,375 expressed sequence tags to nuclear-encoded genes. These sequences, together with 348 brain expressed sequence tags from our previous study, comprise more than 2,500 new human genes and 870,769 base pairs of DNA sequence. These data represent an approximate doubling of the number of human genes identified by DNA sequencing and may represent as many as 5% of the genes in the human genome.