Determination of the reference range of endogenous plasma carnitines in healthy adults
Determination of the reference range of endogenous plasma carnitines in healthy adults
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DOI：
10.1258/acb.2008.008045
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发表时间：
2008-11-01
影响因子：
2.2
通讯作者：
Fornasini, Gianfranco
中科院分区：
文献类型：
--
作者：
Reuter, Stephanie E.;Evans, Allan M.;Fornasini, Gianfranco
背景：左旋肉碱是一种内源性物质，在脂肪酸通过线粒体内膜氧化的运输中至关重要。左旋肉碱体内平衡紊乱会对人体健康产生重大影响；因此，确定左旋肉碱及其酯的正常内源性浓度对左旋肉碱缺乏症的诊断至关重要。本研究采用三种分析方法来确定健康成人中一些肉碱的正常浓度。年龄和性别对肉毒碱浓度的影响也进行了研究。方法：采集60例不同年龄、不同性别的健康人的血液样本。采用放射酶法、高效液相色谱法和电喷雾串联质谱法对血浆样品进行内源性肉毒碱浓度分析。结果：每次测定结果的精密度和准确度均在可接受范围内。本研究三种分析方法得到的左旋肉碱、乙酰左旋肉碱和总左旋肉碱的平均内源浓度分别为38 ~ 44,6 ~ 7和49 ~ 50 μ mol/L。两性比较结果表明，男性内源性血浆左旋肉碱和总左旋肉碱浓度明显高于女性。年龄对血浆肉碱浓度没有影响。结论：这些结果对评价肉碱缺乏症患者的生化或代谢紊乱及诊断和治疗具有重要意义。
Background: L-carnitine is an endogenous substance, vital in the transport of fatty acids across the inner mitochondrial membrane for oxidation. Disturbances in carnitine homeostasis can have a significant impact on human health; therefore, it is critical to define normal endogenous concentrations for L-carnitine and its esters to facilitate the diagnosis of carnitine deficiency disorders. This study was conducted to determine the normal concentrations of a number of carnitines in healthy adults using three analytical methods. The impact of age and gender on carnitine concentrations was also examined.Methods: Blood samples were collected from 60 healthy subjects of both genders and various ages. Plasma samples were analysed for endogenous carnitine concentrations by radioenzymatic assay, high-performance liquid chromatography and electrospray tandem mass spectrometry.Results: Precision and accuracy of results obtained for each assay were within acceptable limits. Average endogenous concentrations obtained from the three analytical methods in this study were in the range of 38-44, 6-7 and 49-50 mu mol/L for L-carnitine, acetyl-L-carnitine and total carnitine, respectively. Comparison of results between the genders indicated that males had significantly higher endogenous plasma L-carnitine and total carnitine concentrations than females. Age was found to have no impact on plasma carnitine concentrations.Conclusion: These results are useful in the evaluation of biochemical or metabolic disturbances and in the diagnosis and treatment of patients with carnitine deficiency.