Poly(oligoethylene glycol methacrylate) Dip-Coating: Turning Cellulose Paper into a Protein-Repellent Platform for Biosensors
Poly(oligoethylene glycol methacrylate) Dip-Coating: Turning Cellulose Paper into a Protein-Repellent Platform for Biosensors
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DOI：
10.1021/ja507372v
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发表时间：
2014-09-17
影响因子：
15
通讯作者：
Hoare, Todd
中科院分区：
文献类型：
--
作者：
Deng, Xudong;Smeets, Niels M. B.;Hoare, Todd
非特异性蛋白质在纸上的钝化吸附是纸作为微流控生物检测平台的主要障碍。在这里，我们描述了一个简单的，可扩展的协议，基于吸附和交联聚（低聚甲基丙烯酸乙二醇酯）（POEGMA）衍生物，减少了一系列蛋白质的非特异性吸附到滤纸至少1个数量级，而不显着改变纤维形态或纸的宏观孔隙度。涂有POEGMA的横向流动测试条促进了有效的蛋白质运输，同时也限制了比色报告信号，使检测更容易，相对于牛血清白蛋白（BSA）阻塞纸，性能得到了改善。基于poegma涂布纸的酶联免疫吸附试验也具有较低的空白值，较高的灵敏度和较低的检测限，相对于基于用BSA或脱脂牛奶阻塞的纸。我们期望poegma涂布纸可以作为设计便携式、一次性和低成本纸质生物传感器的平台。
The passivation of nonspecific protein adsorption to paper is a major barrier to the use of paper as a platform for microfluidic bioassays. Herein we describe a simple, scalable protocol based on adsorption and cross-linking of poly(oligoethylene glycol methacrylate) (POEGMA) derivatives that reduces nonspecific adsorption of a range of proteins to filter paper by at least 1 order of magnitude without significantly changing the fiber morphology or paper macroporosity. A lateral-flow test strip coated with POEGMA facilitates effective protein transport while also confining the colorimetric reporting signal for easier detection, giving improved performance relative to bovine serum albumin (BSA)-blocked paper. Enzyme-linked immunosorbent assays based on POEGMA-coated paper also achieve lower blank values, higher sensitivities, and lower detection limits relative to ones based on paper blocked with BSA or skim milk. We anticipate that POEGMA-coated paper can function as a platform for the design of portable, disposable, and low-cost paper-based biosensors.