Neoadjuvant chemotherapy enhances anti-tumor immune response of tumor microenvironment in human esophageal squamous cell carcinoma
Neoadjuvant chemotherapy enhances anti-tumor immune response of tumor microenvironment in human esophageal squamous cell carcinoma
复制标题
新辅助化疗可增强人食管鳞状细胞癌肿瘤微环境的抗肿瘤免疫反应
DOI：
10.1016/j.isci.2023.106480
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发表时间：
2023-04-06
期刊：
影响因子：
5.8
通讯作者：
Nakamura，Masafumi
中科院分区：
文献类型：
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作者：
Okuda，Sho;Ohuchida，Kenoki;Nakamura，Masafumi
虽然化疗一直是癌症的重要治疗手段，但免疫检查点阻断疗法的发展是革命性的，全面了解免疫肿瘤微环境（TME）已变得至关重要。在这里，我们利用单细胞rna测序研究了新辅助化疗（NAC）对人食管鳞状细胞癌TME免疫细胞的影响。对30个新鲜样本的分析显示，人食管鳞状细胞癌TME中的CD8+/CD4+ T细胞、树突状细胞（dc）和巨噬细胞在NAC（+）组中的抗肿瘤免疫应答水平高于NAC（-）组。此外，NAC（+）组的免疫细胞通过各种细胞因子（包括ifngin CD8+/CD4+ T细胞、EBI3in dc和namptin巨噬细胞）相互作用，导致抗肿瘤免疫反应增强。我们的结果表明NAC可能增强TME中免疫细胞的抗肿瘤免疫反应。
Although chemotherapy has been an essential treatment for cancer, the development of immune checkpoint blockade therapy was revolutionary, and a comprehensive understanding of the immunological tumor microenvironment (TME) has become crucial. Here, we investigated the impact of neoadjuvant chemotherapy (NAC) on immune cells in the TME of human esophageal squamous cell carcinoma using single cell RNA-sequencing. Analysis of 30 fresh samples revealed that CD8+/CD4+ T cells, dendritic cells (DCs), and macrophages in the TME of human esophageal squamous cell carcinoma showed higher levels of an anti-tumor immune response in the NAC(+) group than in the NAC(−) group. Furthermore, the immune cells of the NAC(+) group interacted with each other resulting in enhanced anti-tumor immune response via various cytokines, includingIFNGin CD8+/CD4+ T cells,EBI3in DCs, andNAMPTin macrophages. Our results suggest that NAC potentially enhances the anti-tumor immune response of immune cells in the TME.