Comparison of different solid phase extraction sorbents for the qualitative assessment of dissolved organic nitrogen in freshwater samples using FT-ICR-MS
Comparison of different solid phase extraction sorbents for the qualitative assessment of dissolved organic nitrogen in freshwater samples using FT-ICR-MS
复制标题
使用 FT-ICR-MS 定性评估淡水样品中溶解有机氮的不同固相萃取吸附剂的比较
DOI：
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发表时间：
2018
影响因子：
1.6
通讯作者：
C. Schubert
中科院分区：
文献类型：
--
作者：
Patrick E. Stücheli;J. Niggemann;C. Schubert
溶解有机物 (DOM) 的命运和反应性与其化学成分直接相关。因此，分子表征（例如使用傅里叶变换离子回旋共振质谱 (FT-ICR-MS)）可用于更好地了解这些因素。为了研究水体中的有机化合物，有效的萃取方法非常重要。 FT-ICR-MS 常用的萃取方法是使用反相吸附剂 (BondElut PPL) 的固相萃取 (SPE)。但据我们所知，这种方法并未评估其提取有机氮化合物的能力，有机氮化合物是生命的重要组成部分，因此也是 DOM 的重要组成部分。在这项研究中，测试了几种固相吸附剂从天然水环境的水样中提取有机氮化合物的能力。对不同滤芯的保留机制和孔径进行了测试。测试了具有不同萃取机制（反相、阳离子交换或两者的混合物）或不同孔径的三种滤芯。除了一种吸附剂类型会严重污染样品的有机分子外，测试的滤芯既没有浸出大量的溶解有机碳 (DOC)，也没有浸出大量溶解的有机氮 (DON)。吸附剂用湖水进行了测试，以便能够研究其在实际条件下的功能。可以看出，选择吸附剂材料时应考虑样品的分子组成。此外，研究表明，混床吸附剂是一种有价值的补充 SPE 吸附剂，可用于使用 FT-ICR-MS 对湖相样品进行分子表征，并且也可用于定量萃取。此外，还可以证明，HyperSep Retain CX 吸附剂可以提取更广泛的有机氮化合物，从而为使用 FT-ICR-MS 研究湖泊中的有机氮化合物提供更全面的数据集。
Fate and reactivity of dissolved organic matter (DOM) is directly linked to its chemical composition. Therefore, molecular characterisation, for example using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS), is used for a better understanding of those factors. To study organic compounds in the water column, an efficient extraction method is important. The commonly used extraction method for FT-ICR-MS is solid phase extraction (SPE) using a reversed-phase sorbent (BondElut PPL). But this method, to the best of our knowledge, was not evaluated for its ability to extract organic nitrogen compounds which are important building blocks of life and therefore an important fraction of DOM. In this study, several solid phase sorbents were tested for their ability to extract organic nitrogen compounds from water samples of natural aqueous environments. Different cartridges concerning their retention mechanism and pore size were tested. Three cartridges with different extraction mechanism (reversed phase, cation exchange or a mixture of both) or different pore size were tested. Except for one sorbent type, which heavily contaminated the samples with organic molecules, the tested cartridges leached neither a significant amount of dissolved organic carbon (DOC) nor dissolved organic nitrogen (DON). The sorbents were tested with lake water to be able to investigate their functionality in real conditions. It could be shown, that the molecular composition of the sample should be considered for the choice of the sorbent material. Additionally, it was shown that a mixed-bed sorbent is a valuable complementary SPE sorbent for the molecular characterisation of lacustrine samples using FT-ICR-MS and it might also be useful for a quantitative extraction. Furthermore, it could be shown that HyperSep Retain CX sorbent allows to extract a broader range of organic nitrogen compounds leading to a more comprehensive data set for investigating organic nitrogen compounds in lakes using FT-ICR-MS.
影响因子：
16.6
作者：
Kellerman, Anne M.;Dittmar, Thorsten;Tranvik, Lars J.
通讯作者：
Tranvik, Lars J.
影响因子：
7
作者：
R. Schwartz;Claire Ting;J. King
通讯作者：
R. Schwartz;Claire Ting;J. King
影响因子：
7.4
作者：
Riedel, Thomas;Dittmar, Thorsten
通讯作者：
Dittmar, Thorsten