Dual-beam Fourier domain optical Doppler tomography of zebrafish
Dual-beam Fourier domain optical Doppler tomography of zebrafish
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DOI：
10.1364/oe.16.013624
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发表时间：
2008-09-01
期刊：
影响因子：
3.8
通讯作者：
Ferrante, Anthony A.
中科院分区：
文献类型：
--
作者：
Iftimia, Nicusor V.;Hammer, Daniel X.;Ferrante, Anthony A.
我们开发了双光束傅里叶域光学多普勒断层扫描 (FD-ODT) 系统来对斑马鱼 (Danio rerio) 幼虫进行成像。以固定角度间隔入射到斑马鱼上的两束光束可以进行绝对血流速度测量，无论心脏和大部分主要脉管系统所在的矢状平面中的血管方向如何。两个光谱仪同时从两个干涉仪采集光谱，典型（最大）线速率为 18 (28) kHz。该系统使用稀释牛奶和微球以及 0.5 毫米厚的流通池进行校准。从 1.4 到 34.6 mm/s 的设定速度平均偏差为 4.1%。通过鱼的头部、心脏和上尾部获取整条鱼的三维结构光栅视频。已解决的粗略特征包括端脑、视网膜、两个心室（心房和心室）、鳃弓和脊索。这些器官内的其他精细结构也得到了解决。斑马鱼是高通量筛选新药理学的重要工具。 FD-ODT 能够生成高分辨率三维结构视频并准确测量主要血管中的绝对流速，为研究人员提供了评估新药疗效的额外指标。 (c) 2008 年美国光学学会。
We have developed a dual-beam Fourier domain optical Doppler tomography (FD-ODT) system to image zebrafish (Danio rerio) larvae. Two beams incident on the zebrafish with a fixed angular separation allow absolute blood flow velocity measurement to be made regardless of vessel orientation in a sagittal plane along which the heart and most of the major vasculature lie. Two spectrometers simultaneously acquire spectra from two interferometers with a typical (maximum) line rate of 18 (28) kHz. The system was calibrated using diluted milk and microspheres and a 0.5-mm thick flow cell. The average deviation from the set velocity from 1.4 to 34.6 mm/s was 4.1%. Three-dimensional structural raster videos were acquired of an entire fish, and through the head, heart, and upper tail of the fish. Coarse features that were resolved include the telencephalon, retina, both heart chambers (atrium and ventricle), branchial arches, and notochord. Other fine structures within these organs were also resolved. Zebrafish are an important tool for high-throughput screening of new pharmacological agents. The ability to generate high-resolution three-dimensional structural videos and accurately measure absolute flow rates in major vessels with FD-ODT provides researchers with additional metrics by which the efficacy of new drugs can be assessed. (c) 2008 Optical Society of America.