Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry
Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry
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DOI：
10.1007/s00216-018-1300-7
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发表时间：
2019-01-01
影响因子：
4.3
通讯作者：
Vaculovic, Tomas
中科院分区：
文献类型：
--
作者：
Tvrdonova, Michaela;Vlcnovska, Marcela;Vaculovic, Tomas
在本文中，我们描述了标记抗体的金纳米粒子（AuNPs）的直径为10和60 nm的激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）检测。此外，AuNPs标记策略与基于MeCAT（金属编码亲和标记）的市售标记试剂进行了比较。进行基于斑点印迹实验的原理验证实验。通过灵敏度和检测限（LOD）比较了两种标记方法。所实现的绝对LOD在AuNP标记的数十皮克的范围内，相比之下，MeCAT标记的数百皮克。
In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.