Facile design of reduced graphene oxide decorated with Cu2O nanocube composite as antibiofilm active material
Facile design of reduced graphene oxide decorated with Cu2O nanocube composite as antibiofilm active material
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DOI：
10.1016/j.matchemphys.2019.122300
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发表时间：
2020
影响因子：
4.6
通讯作者：
M. Selim;Nadia A. Samak;Zhifeng Hao;J. Xing
中科院分区：
文献类型：
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作者：
M. Selim;Nadia A. Samak;Zhifeng Hao;J. Xing
通过简单的两相法制备了稳定的还原氧化石墨烯纳米片修饰氧化亚铜纳米立方体（rGO/Cu 2 O）复合材料。使用改进的Hummer方法，随后通过水热还原技术成功地制备了具有0.5- 2nm片厚度的rGO。采用湿化学法在室温下合成了平均直径为70-90 nm、{100}生长方向可控的Cu 2 O纳米立方体，该方法不使用任何有毒且难以清洗的表面活性剂或模板剂。研究了rGO/Cu 2 O纳米复合材料对大肠杆菌、铜绿假单胞菌和枯草芽孢杆菌的抑菌活性和成膜活性，结果表明：rGO/Cu 2 O纳米复合材料对大肠杆菌的最低抑菌浓度（MIC）分别为5.9、2.9和2.9 μg/mL; coli、铜绿假单胞菌（P. aeruginosa）和B.与游离卡那霉素（MIC在23.4-64 μg/mL内）和链霉素（MIC在23.4-187 μg/mL内）相比，分别使用rGO/Cu 2 O纳米复合材料的MIC和更高的MIC值，显著根除了三种测试微生物的生物膜形成和成熟生物膜。扫描电子显微镜阐明了纳米复合材料的抗菌机制，通过完整的包裹细菌细胞和破坏他们的形状形态。我们的研究结果为抗菌纳米材料提供了一个很好的平台，这些材料可以分散形式用于水源中，或者与涂层材料结合以抑制微生物生长和生物膜。
Stable reduced graphene oxide nanosheets decorated with cuprous oxide nanocubes (rGO/Cu2O) composite was fabricated via a facile two-phase method. rGO with 0.5–2 nm sheet thickness was successfully prepared using a modified Hummer's approach followed by a hydrothermal reduction technique. Controlled Cu2O nanocubes with 70–90 nm average diameters and a {100} growth direction were synthesized by a wet chemical technique at room temperature without using any surfactants or templates which are usually toxic and difficult to wash. The antibacterial and antibiofilm activity of rGO/Cu2O nanocomposite was studied towardEscherichia coli,Pseudomonas aeruginosa, andBacillus subtilis.Minimum inhibitory concentration (MIC) values of rGO/Cu2O composite (5.9, 2.9, and 2.9 μg/mL forE. coli,P. aeruginosa, andB. subtilis, respectively) showed higher activities as compared to free kanamycin (MICs within 23.4–64 μg/mL) and streptomycin (MICs within 23.4–187 μg/mL). Biofilm formation and mature biofilm of the three tested microbes were significantly eradicated using MIC and higher-MIC values of the rGO/Cu2O nanocomposite, respectively. Scanning electron microscopy elucidated the antimicrobial mechanism of the nanocomposite through the complete wrapping of the bacterial cells and disrupting their shape morphology. Our findings provide a great platform for antibacterial nano-materials that could be used in water sources in the dispersion form or to be incorporated with coating materials to inhibit microbial growth and biofilms.