Gibberellic Acid, Synthetic Auxins, and Ethylene Differentially Modulate α-l-Arabinofuranosidase Activities in Antisense 1-Aminocyclopropane-1-Carboxylic Acid Synthase Tomato Pericarp Discs1
Gibberellic Acid, Synthetic Auxins, and Ethylene Differentially Modulate α-l-Arabinofuranosidase Activities in Antisense 1-Aminocyclopropane-1-Carboxylic Acid Synthase Tomato Pericarp Discs1
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赤霉酸、合成生长素和乙烯差异调节反义 1-氨基环丙烷-1-羧酸合酶番茄果皮圆盘中 α-l-阿拉伯呋喃糖苷酶活性1
DOI：
10.1104/pp.001180
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发表时间：
2002
期刊：
影响因子：
7.4
通讯作者：
J. Labavitch
中科院分区：
文献类型：
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作者：
G. Sozzi;L. Greve;G. Prody;J. Labavitch
α-l-阿拉伯呋喃糖苷酶（α-Afs）是能够从细胞壁基质聚合物以及从不同的糖缀合物释放末端阿拉伯呋喃糖残基的植物酶。通过对对照番茄（Lycopersicon esculentum）和表达反义1-氨基环丙烷-1-羧酸合酶转基因的乙烯合成抑制（ESS）系的蛋白质提取物进行分子排阻色谱分析，区分了3种不同的α-Af亚型。α-Af I和II在整个果实个体发育过程中具有活性。α-Af I是第一个从番茄果皮组织中分离到的锌依赖性细胞壁酶，表明锌参与了果实细胞壁代谢。这种异构体被1，10-菲咯啉抑制，但在NaCl和蔗糖存在下保持稳定。10 d果实中α-Af Ⅱ活性占总α-Af活性的80%以上，但随着果实成熟，α-Af Ⅱ活性下降。相反，α-Af III是乙烯依赖性的，并且在成熟过程中具有特异性活性。α-Af I从番茄细胞壁的KOH可溶性多糖中释放单糖阿拉伯糖，而α-Af II和III作用于Na 2CO 3可溶性果胶。不同的α-Af异构体响应赤霉酸，合成生长素，乙烯后，使用一种新的ESS成熟绿色番茄果皮圆盘系统。赤霉素和2，4-二氯苯氧乙酸处理提高了α-Af Ⅰ和Ⅱ的活性，而乙烯处理只提高了α-Af Ⅲ的活性。结果表明，番茄α-Afs是由一个受不同激素控制的基因家族编码的，可能具有不同的体内功能。ESS果皮外植体系统允许全面的研究，涉及生理水平的不同的生长调节剂对基因表达和酶活性的影响，可以忽略不计的伤口诱导的乙烯产生。
α-l-Arabinofuranosidases (α-Afs) are plant enzymes capable of releasing terminal arabinofuranosyl residues from cell wall matrix polymers, as well as from different glycoconjugates. Three different α-Af isoforms were distinguished by size exclusion chromatography of protein extracts from control tomatoes (Lycopersicon esculentum) and an ethylene synthesis-suppressed (ESS) line expressing an antisense 1-aminocyclopropane-1-carboxylic synthase transgene. α-Af I and II are active throughout fruit ontogeny. α-Af I is the first Zn-dependent cell wall enzyme isolated from tomato pericarp tissues, thus suggesting the involvement of zinc in fruit cell wall metabolism. This isoform is inhibited by 1,10-phenanthroline, but remains stable in the presence of NaCl and sucrose. α-Af II activity accounts for over 80% of the total α-Af activity in 10-d-old fruit, but activity drops during ripening. In contrast, α-Af III is ethylene dependent and specifically active during ripening. α-Af I released monosaccharide arabinose from KOH-soluble polysaccharides from tomato cell walls, whereas α-Af II and III acted on Na2CO3-soluble pectins. Different α-Af isoform responses to gibberellic acid, synthetic auxins, and ethylene were followed by using a novel ESS mature-green tomato pericarp disc system. α-Af I and II activity increased when gibberellic acid or 2,4-dichlorophenoxyacetic acid was applied, whereas ethylene treatment enhanced only α-Af III activity. Results suggest that tomato α-Afs are encoded by a gene family under differential hormonal controls, and probably have different in vivo functions. The ESS pericarp explant system allows comprehensive studies involving effects of physiological levels of different growth regulators on gene expression and enzyme activity with negligible wound-induced ethylene production.
番茄果实中的 α-L-阿拉伯呋喃糖苷酶
DOI：
--
发表时间：
2007
期刊：
Plant Cell Physiology 48・Suppl
影响因子：
--
作者：
Miyohashi M.;Matsuno J.;Kamiyoshihara Y.;Tateishi A.;Inoue H.
通讯作者：
Inoue H.
DOI：
10.1073/pnas.89.6.2475
发表时间：
1992-03
影响因子：
11.1
作者：
W. Yip;Thomas Moore;S. F. Yang
通讯作者：
W. Yip;Thomas Moore;S. F. Yang