Quantitation and localization of cartilage degeneration following the induction of osteoarthritis in the rabbit knee
Quantitation and localization of cartilage degeneration following the induction of osteoarthritis in the rabbit knee
复制标题
DOI：
10.1016/s1063-4584(97)80039-8
复制
发表时间：
1997-09-01
影响因子：
7
通讯作者：
Sah, RL
中科院分区：
文献类型：
--
作者：
Chang, DG;Iverson, EP;Sah, RL
目的：建立并应用一种新的视频成像技术，对兔单侧前交叉韧带（ACLT）切断诱导骨关节炎后股胫关节软骨的印度墨水染色进行定量和定位。手术后九周，从11个ACLT和对侧对照膝关节采集股骨和胫骨，并定位以获得墨水的校准灰度图像。在90度屈曲时与膝关节相对的涂有油漆的关节软骨表面。对图像进行处理，使得正常软骨区域的反射率得分相对较高，而墨水染色的纤维化软骨和暴露的骨的反射率得分较低。股骨内外侧髁和胫骨平台的比较（MFC、LFC、MTP、对照组和ACLT膝关节的LTP）显示，未被半月板覆盖的MTP区域的反射率评分显著较低（P < 0.001）高于其他地区。ACLT导致总反射率评分下降11%（P < 0.001）。反射率分数下降，作为一个传统的形态学退化分级增加。ACLT诱导的退行性变倾向于关节的后内侧，在较小程度上，前外侧。在胫骨平台，ACLT造成了明显的退化，在覆盖，但不是未覆盖的地区。相对软骨表面的图像分数（即，MFC与MTP和LFC与LTP）显着（R=0.56-0.70，P < 0.001）相关ACLT和对照kneels.Discussion：识别和表征软骨领域容易退化可能是特别有用的生化和生物力学机制在骨关节炎，以及治疗干预的疗效进一步分析。
Objective: To develop and apply a new video imaging technique to quantify and localize Indian ink staining of cartilage of the rabbit femorotibial joint after the induction of osteoarthritis by unilateral transection of the anterior cruciate ligament (ACLT).Methods: Nine weeks after surgery, femora and tibiae from 11 ACLT and contralateral control knees were harvested and positioned to obtain calibrated gray-scale images of the ink-painted articular cartilage surfaces that are opposed with the knee in 90 degrees flexion. Images were processed so that areas of normal cartilage gave a relatively high reflectance score, whereas ink-stained fibrillated cartilage and exposed bone gave low scores.Results: Comparison of the medial and lateral femoral condyles and tibial plateaus (MFC, LFC, MTP, LTP) of control and ACLT knees showed that the area of the MTP not covered by the meniscus had a significantly lower reflectance score (P < 0.001) than other areas. ACLT led to an 11% decrease (P < 0.001) in the overall reflectance score. The reflectance score decreased as a traditional morphological grading of degeneration increased. ACLT-induced degeneration had a predilection for the posteromedial aspects of the joint, and to a lesser extent, the anterolateral aspects. In the tibial plateaus, ACLT caused significant degeneration in the covered, but not the uncovered, areas. Image scores of opposing cartilage surfaces (i.e., MFC vs MTP and LFC vs LTP) were significantly (R=0.56-0.70, P < 0.001) correlated in ACLT and control knees.Discussion: Identification and characterization of cartilage areas prone to degeneration may be particularly useful for further analysis of biochemical and biomechanical mechanisms in osteoarthritis, as well as the efficacy of therapeutic interventions.