Organization of the neurofascin gene and analysis of developmentally regulated alternative splicing
Organization of the neurofascin gene and analysis of developmentally regulated alternative splicing
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DOI：
10.1074/jbc.272.45.28742
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发表时间：
1997-11-07
影响因子：
4.8
通讯作者：
Volkmer, H
中科院分区：
文献类型：
--
作者：
Hassel, B;Rathjen, FG;Volkmer, H
神经束蛋白是免疫球蛋白超家族L1亚群的轴突成员，与胚胎发育过程中的神经突延伸有关。在这里，我们分离并表征了鸡神经束蛋白的编码基因。基因组序列与cDNA序列的比较提供了内含子/外显子边界的结构和定位，并表明神经束蛋白同种异构体是通过其前mrna的选择性剪接产生的。神经束蛋白基因由分布在72千碱基上的33个外显子组成。6个免疫球蛋白和5个纤维连接蛋白iii型结构域中的每一个都由两个外显子编码。结构域之间的内含子属于第1阶段，其他的则属于第0、1或2阶段。聚合酶链反应分析表明，神经束蛋白的选择性剪接受发育调节。此外，利用长链聚合酶链反应扩增的神经束蛋白cDNA质粒文库来检测和量化单个神经束蛋白分子中选择性剪接外显子的分布；我们找到了50个。不同的神经束蛋白亚型在不同的发育阶段，并揭示了一个主要的“早期”与多个“晚期”神经束蛋白亚型的存在。
Neurofascin is an axonal member of the L1 subgroup of the immunoglobulin superfamily implicated in neurite extension in the course of embryonic development. Here we have isolated and characterized the gene encoding chicken neurofascin. Comparison of genomic sequences with cDNA sequences provides the structure and localization of intron/exon boundaries and indicates that neurofascin isoforms are generated by alternative splicing of its pre-mRNA. The neurofascin gene is composed of 33 exons distributed over 72 kilobases. Each of the six immunoglobulin- and five fibronectin-type III-like domains is encoded by two exons. While introns between domains are of phase 1, others are of phase 0, 1, or 2. Alternative splicing of neurofascin is developmentally regulated as shown by polymerase chain reaction analysis. Furthermore, plasmid libraries from long range polymerase chain reaction-amplified cDNA of neurofascin were used to examine and quantify the distribution of alternatively spliced exons in individual neurofascin molecules; We found 50. different neurofascin isoforms at different developmental stages and revealed the existence of one major ''early'' in comparison with multiple ''late'' neurofascin isoforms.