The phage abortive infection system, ToxIN, functions as a protein-RNA toxin-antitoxin pair
The phage abortive infection system, ToxIN, functions as a protein-RNA toxin-antitoxin pair
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DOI：
10.1073/pnas.0808832106
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发表时间：
2009-01-20
影响因子：
11.1
通讯作者：
Salmond, George P. C.
中科院分区：
文献类型：
--
作者：
Fineran, Peter C.;Blower, Tim R.;Salmond, George P. C.
存在使细菌能够抵抗噬菌体感染的各种机制。抗性策略包括流产感染（Abi）系统，其促进细胞死亡并限制细菌群体内的噬菌体复制。描述了一种来自植物病原体胡萝卜软腐欧文氏菌（Erwiniacarotovora）亚种atroseptica的高效双基因Abi系统，命名为ToxIN。ToxIN Abi系统还作为毒素-抗毒素（TA）对发挥作用，其中ToxN抑制细菌生长，而串联重复的ToxI RNA抗毒素抵消毒性。TA模块目前分为2类，蛋白质和RNA反义。我们提供的证据表明，毒素定义了一个全新的TA类，通过一种新的蛋白质-RNA机制发挥作用，在不同的细菌中存在类似的系统。尽管TA系统的作用存在争议，但我们证明了毒素在一系列细菌属中提供了对多种细菌的病毒抗性。这是第一次演示一种新的机械类TA系统和一个阿比系统在不同的细菌属中发挥作用，无论是与噬菌体-细菌相互作用的动力学的影响。
Various mechanisms exist that enable bacteria to resist bacteriophage infection. Resistance strategies include the abortive infection (Abi) systems, which promote cell death and limit phage replication within a bacterial population. A highly effective 2-gene Abi system from the phytopathogen Erwinia carotovora subspecies atroseptica, designated ToxIN, is described. The ToxIN Abi system also functions as a toxin-antitoxin (TA) pair, with ToxN inhibiting bacterial growth and the tandemly repeated ToxI RNA antitoxin counteracting the toxicity. TA modules are currently divided into 2 classes, protein and RNA antisense. We provide evidence that ToxIN defines an entirely new TA class that functions via a novel protein-RNA mechanism, with analogous systems present in diverse bacteria. Despite the debated role of TA systems, we demonstrate that ToxIN provides viral resistance in a range of bacterial genera against multiple phages. This is the first demonstration of a novel mechanistic class of TA systems and of an Abi system functioning in different bacterial genera, both with implications for the dynamics of phage-bacterial interactions.