Effects of Exposure to Zearalenone on Porcine Oocytes and Sperm During Maturation and Fertilization In Vitro
Effects of Exposure to Zearalenone on Porcine Oocytes and Sperm During Maturation and Fertilization In Vitro
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DOI：
10.1262/jrd.11-026h
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发表时间：
2011-08-01
影响因子：
1.8
通讯作者：
Fink-Gremmels, Johanna
中科院分区：
文献类型：
--
作者：
Sambuu, Rentsenkhand;Takagi, Mitsuhiro;Fink-Gremmels, Johanna
研究了玉米赤霉烯酮（ZEN）急性暴露对猪卵母细胞体外成熟和受精过程中精子穿透能力的影响。首先，将卵母细胞在含ZEN（0-1000 μ g/l）的成熟培养基中培养，然后受精。然后将在没有ZEN的情况下体外成熟的卵母细胞在含有ZEN的受精培养基中受精。当ZEN浓度为1000 μ g/l时，卵母细胞的成熟率和精子的穿透能力均显著降低。然而，既没有增加的退化率和DNA片段化的卵母细胞，也没有减少正常和多精受精。ZEN不影响精子穿透率;然而，1000 μ g/l ZEN对正常和多精受精率有积极影响。因此，可以认为，猪卵母细胞在成熟过程中以及卵母细胞和精子在受精过程中急性暴露于高达1000 μ g/l的ZEN可能不会影响卵母细胞的生育力。
The influence of acute exposure to zearalenone (ZEN) on porcine oocyte maturation, fertilization or sperm penetration ability during both in vitro maturation and fertilization was evaluated. First, oocytes were cultured in ZEN-containing (0-1000 mu g/l) maturation medium and then fertilized. The oocytes maturing in vitro without ZEN were then fertilized in ZEN-containing fertilization medium. The maturation rates of oocytes and penetration ability of sperm decreased significantly in the presence of 1000 mu g/l of ZEN. However, neither increases in the rates of degeneration and DNA fragmentation of oocytes nor reductions in normal and polyspermic fertilization were observed. ZEN did not affect the sperm penetration rates; however, 1000 mu g/l ZEN had positive effects on normal and polyspermic fertilization rates. Therefore, it can be suggested that an acute exposure of porcine oocytes during maturation and of oocytes and sperm during fertilization to ZEN up to 1000 mu g/l may not affect the fertility of the oocytes.