Glutathione peroxidase 1 is regulated by the c-Abl and Arg tyrosine kinases
Glutathione peroxidase 1 is regulated by the c-Abl and Arg tyrosine kinases
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DOI：
10.1074/jbc.m305770200
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发表时间：
2003-10-10
影响因子：
4.8
通讯作者：
Kufe, D
中科院分区：
文献类型：
--
作者：
Cao, C;Leng, YM;Kufe, D
c-Abl和精氨酸酪氨酸激酶在细胞对氧化应激的反应中被激活。目前的研究表明，c-Abl和Arg与谷胱甘肽过氧化物酶1 （GPx1）相关，这种相互作用受细胞内氧化剂水平的调节。c-Abl和Arg SH3结构域直接与GPx1中富含脯氨酸的位点在132 - 145个氨基酸上结合。GPx1还作为c-Abl和arg介导的tyr1 -96磷酸化的底物。结果进一步表明，c-Abl和Arg刺激GPx活性，这些激酶有助于GPx介导的细胞抗氧化应激保护。我们的研究结果首次证明GPx1受氧化应激反应中激活的信号通路调控。
The c-Abl and Arg tyrosine kinases are activated in the cellular response to oxidative stress. The present studies demonstrate that c-Abl and Arg associate with glutathione peroxidase 1 (GPx1) and that this interaction is regulated by intracellular oxidant levels. The c-Abl and Arg SH3 domains bind directly to a proline-rich site in GPx1 at amino acids 132 - 145. GPx1 also functions as a substrate for c-Abl- and Arg-mediated phosphorylation on Tyr-96. The results further show that c-Abl and Arg stimulate GPx activity and that these kinases contribute to GPx-mediated protection of cells against oxidative stress. Our findings provide the first evidence that GPx1 is regulated by a signaling pathway that is activated in the oxidative stress response.