Adipose-derived mesenchymal stromal cells prevented rat vocal fold scarring
Adipose-derived mesenchymal stromal cells prevented rat vocal fold scarring
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DOI：
10.1002/lary.26855
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发表时间：
2018-01-01
期刊：
影响因子：
2.6
通讯作者：
Hirano, Shigeru
中科院分区：
文献类型：
--
作者：
Morisaki, Tsuyoshi;Kishimoto, Yo;Hirano, Shigeru
目的/假设本研究旨在通过研究脂肪间充质基质细胞（adipose-derived mesenchymal stromal cells，ASCs）移植到受损大鼠声带中对基因转录和翻译水平的影响，揭示ASCs预防声带瘢痕形成的作用。SD大鼠剥除声带后，立即向甲杓肌内注射ASCs（对照组）或生理盐水（假手术组）。对于组织学检查，在注射后3、14和56天摘除喉。实时定量聚合酶链反应（PCR）分析进行了3和14天后injection.ResultsTransplanted ASCs检测仅在喉在第3天。在第14天和第56天，组织学检查显示，与假手术组相比，ASCs组中透明质酸的量显著更高，胶原的沉积更低。Real-time PCR检测结果显示，ASCs组各时间点前胶原（Col）1a 1、Col 1a 3、基质金属蛋白酶（Mmp）1和Mmp 8均呈低表达。与假手术组相比，ASCs组在第14天成纤维细胞生长因子（Fgf）2和肝细胞生长因子（Hgf）的表达明显增加。结论ASCs可增加Fgf 2和Hgf的表达，抑制声带创伤愈合过程中胶原的过度沉积。鉴于ASC存活不超过14天的事实，ASC被认为诱导周围细胞中生长因子基因的上调。这些结果表明，ASCs有可能防止声带瘢痕形成。喉镜，128：E33-E40，2018
Objectives/HypothesisThis study aimed to reveal the effects of adipose-derived mesenchymal stromal cells (ASCs) on prevention of vocal fold scarring by investigating how the immediate ASCs transplantation into the injured rat vocal fold affect the levels of gene transcription and translation.Study DesignProspective animal experiments with controls.MethodsASCs harvested from green fluorescent protein transgenic rat (ASCs group) or saline (sham group) were injected into the thyroarytenoid muscle of Sprague-Dawley rats immediately after stripping the vocal fold. For histological examinations, larynges were extirpated at 3, 14, and 56 days after the injection. Quantitative real-time polymerase chain reaction (PCR) analyses were performed at 3 and 14 days after the injection.ResultsTransplanted ASCs were detected only in larynges at day 3. At days 14 and 56, histological examination showed significantly higher amounts of hyaluronic acid and lower deposition of collagen in the ASCs group compared to the sham group. Real-time PCR revealed that the ASCs group showed low expression of procollagen (Col)1a1, Col1a3, matrix metalloproteinase (Mmp)1 and Mmp8 in each time points. The ASCs group showed high expression of fibroblast growth factor (Fgf)2 and Hepatocyte growth factor (Hgf) compared to the sham group at day 14.ConclusionsASCs increased expressions of Fgf2 and Hgf, and suppressed excessive collagen deposition during vocal fold wound healing. Given the fact that ASCs survived no more than 14 days, ASCs were thought to induce upregulations of growth factors' genes in surrounding cells. These results suggested that ASCs have potential to prevent vocal fold scarring.Level of EvidenceNA. Laryngoscope, 128:E33-E40, 2018