Method for obtaining structure and interactions from oriented lipid bilayers
Method for obtaining structure and interactions from oriented lipid bilayers
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DOI：
10.1103/physreve.63.011907
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发表时间：
2001-01-01
影响因子：
2.4
通讯作者：
Nagle, JF
中科院分区：
文献类型：
--
作者：
Lyatskaya, Y;Liu, YF;Nagle, JF
精确计算的散射强度1（q）从一个取向的脂质双层使用一个现实的波动模型的堆栈。感兴趣的量包括双层弯曲模量K-c、双层间相互作用模量B和通过形状因子F（q（z））的双层结构。它示出了如何K-c和B可以从大q（z）的波动占主导地位的数据获得。通过在远离峰值的q区域中使用I（q），并且对于不等于0的q（r），可以在q（z）的宽范围内对F（q（z））进行良好的估计，其中散射域的细节起的作用很小。域大小的粗略估计也可以从较小的q（z）数据进行。结果显示了在L-α相中完全水合的定向DOPC双层上采集的数据。这些结果说明了与粉末样品相比，取向样品的优点。
Precise calculations are made of the scattering intensity 1(q) from an oriented stack of lipid bilayers using a realistic model of fluctuations. The quantities of interest include the bilayer bending modulus K-c, the interbilayer interaction modulus B, and bilayer structure through the form factor F(q(z)). It is shown how K-c and B may be obtained from data at large q(z) where fluctuations dominate. Good estimates of F(q(z)) can be made over wide ranges of q(z) by using I(q) in q regions away from the peaks and for q(r)not equal0 where details of the scattering domains play little role. Rough estimates of domain sizes can also be made from smaller q(z) data. Results are presented for data taken on fully hydrated, oriented DOPC bilayers in the L-alpha phase. These results illustrate the advantages of oriented samples compared to powder samples.