Gonadal steroidogenesis in vitro from juvenile alligators obtained from contaminated or control lakes.
Gonadal steroidogenesis in vitro from juvenile alligators obtained from contaminated or control lakes.
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DOI：
10.1289/ehp.95103s431
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发表时间：
1995-05
影响因子：
10.4
通讯作者：
Percival HF
中科院分区：
文献类型：
--
作者：
Guillette LJ Jr;Gross TS;Gross DA;Rooney AA;Percival HF
许多污染物的无处不在的分布和非致命的，多代的影响，这些污染物对生殖，内分泌和免疫系统，导致人们担心，世界各地的野生动物受到影响。虽然致病因子和影响是已知的一些物种，与污染物诱导的生殖修饰相关的潜在生理机制仍然知之甚少，需要广泛的研究。我们描述了一项研究，检查类固醇激素的活性，从少年短吻鳄（短吻鳄密西西比）从污染或控制湖泊在中央佛罗里达。雌二醇-17 β（E2）的合成显着不同时，卵巢从污染和控制湖泊进行了比较，在体外。此外，从受污染的湖泊中获得的男性睾丸。湖Apopka，合成显着更高浓度的E2相比，从控制男性的睾丸。与此相反，睾酮（T）的合成，从所有的睾丸在这项研究中显示了正常的模式，并产生浓度大于从卵巢中观察到的任何湖泊。有趣的是，性腺类固醇生成的模式不同于先前报道的从相同个体获得的这些激素的血浆浓度。我们认为，在体内和体外模式之间的差异是由于血浆性类固醇激素的肝降解的修改。
The ubiquitous distribution of many contaminants and the nonlethal, multigenerational effects of such contaminants on reproductive, endocrine, and immune systems have led to concerns that wildlife worldwide are affected. Although the causal agents and effects are known for some species, the underlying physiological mechanisms associated with contaminant-induced reproductive modifications are still poorly understood and require extensive research. We describe a study examining the steroidogenic activity of gonads removed from juvenile alligators (Alligator mississippiensis) obtained from contaminated or control lakes in central Florida. Synthesis of estradiol-17 beta (E2) was significantly different when ovaries from the contaminated and control lakes were compared in vitro. Additionally, testes from males obtained from the contaminated lake. Lake Apopka, synthesized significantly higher concentrations of E2 when compared to testes obtained from control males. In contrast, testosterone (T) synthesis from all testes examined in this study displayed a normal pattern and produced concentrations greater than that observed from ovaries obtained from either lake. Interestingly, the pattern of gonadal steroidogenesis differs from previously reported plasma concentrations of these hormones obtained from the same individuals. We suggest that the differences between the in vivo and in vitro patterns are due to modifications in the hepatic degradation of plasma sex steroid hormones.