Functional metabolomics approach reveals the reduced biosynthesis of fatty acids and TCA cycle is required for pectinase activity in Bacillus licheniformis
Functional metabolomics approach reveals the reduced biosynthesis of fatty acids and TCA cycle is required for pectinase activity in Bacillus licheniformis
复制标题
功能代谢组学方法揭示了地衣芽孢杆菌中脂肪酸生物合成的减少和果胶酶活性所需的 TCA 循环
DOI：
10.1007/s10295-018-2071-z
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发表时间：
2018-11-01
影响因子：
3.4
通讯作者：
Ye, Xiuyun
中科院分区：
文献类型：
--
作者：
Guan, Yi;Yin, Di;Ye, Xiuyun
果胶酶活性的提高在发酵工业中尤为重要。了解果胶酶的代谢机制，可以找到促进果胶酶高产的代谢调控途径。两株地衣芽孢杆菌DY 1的果胶酶活力均高于DY 2。基于GC-MS的代谢组学鉴定了DY 2与DY 1相比的差异代谢组学，表征了增加的TCA循环和脂肪酸的生物合成。丙酮酸脱氢酶（PDH）、α-酮基葡萄糖脱氢酶（KGDH）和琥珀酸脱氢酶（SDH）活性的升高表明碳代谢的整体提高，这与DY 2比DY 1降低葡萄糖的结果一致。抑制剂丙二酸，糠醛和三氯生，PDH，SDH和脂肪酸的生物合成，促进果胶酶活性，其中三氯生增加果胶酶活性的179%。这些结果表明，功能代谢组学是了解发酵生产代谢机制的有效途径，并为开发改变细菌生理和生产的新方法提供线索。
Increase of pectinase activity is especially important in fermentation industry. Understanding of the metabolic mechanisms can find metabolic modulation approach to promote high yield of pectinase. Higher activity of pectinase was detected in DY1 than DY2, two strains of Bacillus licheniformis. GC–MS-based metabolomics identified differential metabolome of DY2 compared with DY1, characterizing the increased TCA cycle and biosynthesis of fatty acids. Elevated activity of pyruvate dehydrogenase (PDH), α-ketoglutaric dehydrogenase (KGDH) and succinate dehydrogenase (SDH) showed global elevation of carbon metabolism, which is consistent with the result that lowers glucose in DY2 than DY1. Inhibitors malonate, furfural and triclosan, of PDH, SDH and biosynthesis of fatty acids, promoted pectinase activity, where triclosan increased pectinase activity by 179%. These results indicate that functional metabolomics is an effective approach to understand metabolic mechanisms of fermentation production and provides clues to develop new methods for changing bacterial physiology and production.