Synaptic Connectivity between the Cortex and Claustrum Is Organized into Functional Modules
Synaptic Connectivity between the Cortex and Claustrum Is Organized into Functional Modules
复制标题
DOI：
10.1016/j.cub.2020.05.031
复制
发表时间：
2020-07-20
期刊：
影响因子：
9.2
通讯作者：
Silberberg, Gilad
中科院分区：
文献类型：
--
作者：
Chia, Zach;Augustine, George J.;Silberberg, Gilad
屏状核和新皮层之间广泛的相互连接刺激了许多关于其功能的假设;所有这些都表明，屏状核作为一个枢纽，通过密集的相互突触通路连接多个皮层区域。尽管前扣带皮层（ACC）和屏状核之间的连接被认为是自上而下的认知控制的重要途径，但我们对驱动屏状核细胞投射到ACC的突触输入知之甚少。和小鼠屏状核的光遗传学研究ACC投射屏状核（CLA-ACC）神经元的皮质输入和输出。发现同侧和对侧皮层区域都向CLA-ACC神经元提供突触输入。这些皮层区域主要是额叶和边缘区，而不是主要的感觉运动区。我们发现，CLA-ACC神经元从岛叶皮层接受单突触输入，从而揭示了一个潜在的屏状核基质介导的显着性网络。相反，感觉运动皮层区域优先针对非CLA-ACC屏状核神经元。使用双逆行标记的屏状核投射神经元，我们显示选择性也在CLA-ACC神经元的皮质目标：而CLA-ACC神经元主要共同投射到其他额叶区域，屏状核神经元投射到初级感觉运动皮质选择性地针对其他感觉运动区域。我们的研究结果表明，皮质输入和预测从CLA-ACC神经元是高度选择性的，这表明组织的皮质claustral连接到功能模块，可以专门用于处理不同类型的信息。
The widespread reciprocal connectivity between the claustrum and the neocortex has stimulated numerous hypotheses regarding its function; all of these suggest that the claustrumacts as a hub that connects multiple cortical regions via dense reciprocal synaptic pathways. Although the connectivity between the anterior cingulate cortex (ACC) and the claustrum has been proposed as an important pathway for top-down cognitive control, little is known about the synaptic inputs that drive claustrum cells projecting to the ACC. Here, we used multi-neuron patch clamp recordings, retrograde and anterograde viral labeling, and optogenetics in mouse claustrumto investigate cortical inputs and outputs of ACC-projecting claustrum (CLA-ACC) neurons. Both ipsilateral and contralateral cortical regions were found to provide synaptic input to CLA-ACC neurons. These cortical regions were predominantly frontal and limbic regions and not primary sensorimotor regions. We show that CLA-ACC neurons receive monosynaptic input from the insular cortex, thereby revealing a potential claustrum substrate mediating the Salience Network. In contrast, sensorimotor cortical regions preferentially targeted non CLA-ACC claustrum neurons. Using dual retrograde labeling of claustrum projection neurons, we show selectivity also in the cortical targets of CLA-ACC neurons: whereas CLA-ACC neurons co-projected mainly to other frontal regions, claustrum neurons projecting to primary sensorimotor cortices selectively targeted other sensorimotor regions. Our results show that both cortical inputs to and projections from CLA-ACC neurons are highly selective, suggesting an organization of cortico-claustral connectivity into functional modules that could be specialized for processing different types of information.