Expression and Correlation of Lewis y Antigen and Integrins α5 and β1 in Ovarian Serous and Mucinous Carcinoma
Expression and Correlation of Lewis y Antigen and Integrins α5 and β1 in Ovarian Serous and Mucinous Carcinoma
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DOI：
10.1111/igc.0b013e3181ea7ecb
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发表时间：
2010-12-01
影响因子：
4.8
通讯作者：
Zhang, Shulan
中科院分区：
文献类型：
--
作者：
Li, Quanrong;Liu, Shuice;Zhang, Shulan
简介：本研究探讨了Lewis y和整合素α(5)和β(1)在浆液性和粘液性卵巢肿瘤中的表达及其临床意义，然后评估了它们之间的关联。方法：在恶性、交界性和良性卵巢浆液性和粘液性肿瘤以及正常组织中检测Lewis y和整合素α(5)和β(1)的表达。采用免疫组化和双标免疫荧光法在石蜡切片中评估其表达及关系。结果：Lewis y主要表达于卵巢浆液性和粘液性癌（88.33%）；其阳性率明显高于交界性肿瘤（60.00%，P < 0.05）、良性卵巢肿瘤（35.00%，P < 0.01）和正常卵巢组织（0，P < 0.01），且与临床病理特征无关。整合素α（5）（85.00％）和β（1）（81.67％）也主要在卵巢浆液性和粘液性癌中表达；其阳性率均明显高于卵巢良性肿瘤（分别为60.00%和55.00%，均P < 0.05）和正常组织（分别为40.00%和30.00%，均P < 0.01）。整合素α(5)和β(1)表达增加与较高的临床分期相关(P < 0.05)，但与组织学类型、分化程度和淋巴转移无关(P < 0.05)。 Lewis y和整合素α(5)和β(1)在卵巢癌中的表达强度与临床分期和分化程度具有显着性差异(均P < 0.05)。在浆液性和粘液性卵巢癌组织中，Lewis y抗原与整合素α(5)和β(1)呈显着正相关。结论：Lewis y、整合素α(5)和β(1)与卵巢癌之间存在密切相关性。 Lewis y作为整合素α(5)和β(1)的重要组成部分，可以通过促进细胞粘附和迁移等信号通路影响肿瘤细胞的生物学行为，该研究为卵巢癌生物治疗提供了理论依据。
Introduction: This study investigates the expression and the clinical significance of Lewis y and integrins alpha(5) and beta(1) in serous and mucinous ovarian tumors and then evaluates the association between them.Methods: Lewis y and integrin alpha(5) and beta(1) expression are detected on tissues from malignant, borderline, and benign ovarian serous and mucinous tumors and normal tissues. Their expression and relationship are assessed in paraffin sections using immunohisto-chemistry and double-labeling immunofluorescence method.Results: Lewis y was mainly expressed in ovarian serous and mucinous cancers (88.33%); its positive rate was obviously higher than rates in the borderline (60.00%, P < 0.05) and benign ovarian tumors (35.00%, P < 0.01) and normal ovarian tissues (0, P < 0.01) and was not associated with clinicopathological characteristics. Integrins alpha(5) (85.00%) and beta(1) (81.67%) were also mainly expressed in ovarian serous and mucinous cancers; their positive rates were all obviously higher than those in benign ovarian tumors (60.00% and 55.00%, respectively; all P < 0.05) and normal tissues (40.00% and 30.00%, respectively; all P < 0.01). Increased expression of integrins alpha(5) and beta(1) correlated with higher clinical stage (P < 0.05) but were not associated with histological types, differentiation degree, and lymphatic metastasis (P < 0.05). The expression intensity of Lewis y and integrins alpha(5) and beta(1) was significant with clinical stage and differentiation degree (all P < 0.05) in ovarian cancer; positive significant correlation between Lewis y antigen and integrins alpha(5) and beta(1) was observed in serous and mucinous ovarian cancer tissues.Conclusions: A close correlation between Lewis y, integrins alpha(5) and beta(1), and ovarian cancer was observed. Lewis y can influence the biological behavior of a tumor cell as an important composition of integrins alpha(5) and beta(1) by some signal pathway, such as promoting cell adhesion and migration, and this study provides theoretical evidence of ovarian cancer biological treatment.