Electrochemical control of glucose oxidase-catalyzed redox reaction using an oil/water interface
Electrochemical control of glucose oxidase-catalyzed redox reaction using an oil/water interface
复制标题
DOI：
10.1039/b400338a
复制
发表时间：
2004-07
影响因子：
3.3
通讯作者：
T. Sugihara;H. Hotta;T. Osakai
中科院分区：
文献类型：
--
作者：
T. Sugihara;H. Hotta;T. Osakai
用循环伏安法研究了葡萄糖氧化酶（GOD）催化硝基苯（NB）和葡萄糖水溶液（W）中的电子转移反应。当采用电中性化合物四氯苯醌（CQ）作为NB的氧化剂时，由于CQ从NB到W的自发转移，酶促反应不能被调节。在这种情况下，对于酶催化的电子转移观察到的伏安波根据直到电压扫描开始的静置时间而增加。然而，当采用离子氧化剂二甲基铁离子（DiMFc+）作为氧化剂时，通过控制DiMFc+的界面转移来实现酶促反应的电化学控制，从而对于不同浓度的DiMFc+和不同的扫描速率可以获得良好重现的伏安图。的伏安行为成功地解释了基于离子转移机制，其中涉及的界面转移的DiMFc+和随后的GOD催化的电子转移，发生在界面上不均匀，但均匀的W相的基础上的数字模拟。
Glucose oxidase (GOD)-catalyzed electron transfers between some oxidants in nitrobenzene (NB) and glucose in water (W) were studied by cyclic voltammetry. When an electrically neutral compound, chloranil (CQ), was employed as the oxidant in NB, the enzymatic reaction could not be regulated because of the spontaneous transfer of CQ from NB to W. In this case, the voltammetric wave observed for the enzyme-catalyzed electron transfer was increased depending on the standing time until the voltage scan was started. However, when an ionic oxidant, dimethylferricenium ion (DiMFc+), was employed as the oxidant, the electrochemical control of the enzymatic reaction was achieved by controlling the interfacial transfer of DiMFc+, so that well-reproducible voltammograms could be obtained for different concentrations of DiMFc+ and for different scan rates. The voltammetric behaviors were successfully explained by a digital simulation based on the ion-transfer mechanism, which involves the interfacial transfer of DiMFc+ and the succeeding GOD-catalyzed electron transfer which occurs not heterogeneously at the interface, but homogeneously in the W phase.