Uptake and Killing of Leptospira interrogans and Borrelia burgdorferi, Spirochetes Pathogenic to Humans, by Reticuloendothelial Cells in Perfused Rat Liver
Uptake and Killing of Leptospira interrogans and Borrelia burgdorferi, Spirochetes Pathogenic to Humans, by Reticuloendothelial Cells in Perfused Rat Liver
复制标题
灌注大鼠肝脏中的网状内皮细胞摄取并杀死问号钩端螺旋体和伯氏疏螺旋体（对人类致病的螺旋体）
DOI：
10.1128/iai.68.9.5408-5411.2000
复制
发表时间：
2000
影响因子：
3.1
通讯作者：
R. Cevenini
中科院分区：
文献类型：
--
作者：
A. Marangoni;R. Aldini;V. Sambri;M. Montagnani;G. Ballardini;E. Storni;R. Cevenini
在原位灌注的大鼠肝脏中注入单剂量的1.5 × 107个放射性标记的钩端螺旋体（钩端螺旋体病病原体）或莱姆病病原体伯氏疏螺旋体IRS的问号血清型黄疸出血型钩端螺旋体细胞。肝摄取L.疑问句和B。对L. burgdorferi的摄取率为37.4% ± 2.3%。而B型为60.5% ± 3.1%。burgdorferi。与疏螺旋体相反，当肝脏样品在生长培养基中培养时，从肝脏中回收钩端螺旋体。输注后30分钟内，在胆汁中回收到钩端螺旋体，但未回收到疏螺旋体。钩端螺旋体和疏螺旋体与肝脏网状内皮细胞的协会被证明是由免疫组织化学。钩端螺旋体和疏螺旋体被发现与波形蛋白阳性细胞，而不是结蛋白阳性细胞。少数钩端螺旋体，但没有疏螺旋体也被认为与波形蛋白和结蛋白阴性细胞，表明肝窦腔外的空间，在肝实质中的钩端螺旋体的存在。
ABSTRACT In situ-perfused rat livers were infused with a single dose of 1.5 × 107 radiolabeled cells of Leptospira interrogans serovar icterohaemorrhagiae, the agent of leptospirosis, or with Borrelia burgdorferi IRS, the agent of Lyme disease. Significant (P < 0.0001) differences in the liver uptake of L. interrogans and of B. burgdorferi were observed, the uptakes being 37.4% ± 2.3% for L. interrogans and 60.5% ± 3.1% for B. burgdorferi. Leptospires, in contrast to borreliae, were recovered from the livers when liver samples were cultured in growth medium. Leptospires but not borreliae were recovered in bile within 30 min of infusion. The association of leptospires and borreliae with reticuloendothelial cells of the liver was demonstrated by immunohistochemistry. Leptospires and borreliae were found to be associated with vimentin-positive cells and not with desmin-positive cells. Few leptospires but no borreliae were also seen associated with vimentin- and desmin-negative cells, suggesting the presence of leptospires outside the sinusoidal spaces, in the liver parenchyma.